An Oral Glucose Load Decreases Postprandial Extracellular Vesicles in Obese Adults with and without Prediabetes
An Oral Glucose Load Decreases Postprandial Extracellular Vesicles in Obese Adults with and without Prediabetes
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DOI：
10.3390/nu11030580
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发表时间：
2019-03-08
期刊：
影响因子：
5.9
通讯作者：
Malin, Steven K.
中科院分区：
文献类型：
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作者：
Eichner, Natalie Z. M.;Gilbertson, Nicole M.;Malin, Steven K.
尽管细胞外囊泡 (EV) 是 2 型糖尿病 (T2D) 和心血管疾病 (CVD) 的新型生物介质，但高血糖对人类 EV 的影响尚不清楚。我们测试了以下假设：75 克口服葡萄糖耐量试验 (OGTT) 会促进与 CVD 风险相关的 EV 变化。使用美国糖尿病协会标准（75 g OGTT 和/或 HbA1c）对 25 名肥胖成人（年龄：52.4 +/- 3.2 岁，BMI：32.5 +/- 1.2 kg/m(2)）进行正常糖耐量（NGT，n = 8）和糖尿病前期（PD，n = 17）筛查。测量身体成分（生物电阻抗）和健康度（VO(2)峰值）。在 0、60 和 120 分钟时测量动脉僵硬度（增强指数；AIx），同时在 OGTT 期间每 30 分钟评估一次胰岛素、葡萄糖和游离脂肪酸，以评估 CVD 风险。膜联蛋白 V 阳性（AV+）和膜联蛋白 V 阴性（AV-）总 EV、血小板 EV（CD31（+）/CD41（+）；CD41（+））、白细胞 EV（CD45（+）；CD45（+）/CD41（-））、血小板内皮细胞粘附分子（PECAM）（CD31（+））和内皮细胞 EV（CD） 31(+)/CD41(-)；CD105(+))在0和120分钟时收集。 NGT 和 PD 之间的年龄、BMI 或体脂没有差异（均 P > 0.63）。 OGTT 后总 EV、AV+ CD31(+) (PECAM) 和 AV+ CD31(+)/CD41(-)（内皮） EV 减少（P
Although extracellular vesicles (EVs) are a novel biomediator of type 2 diabetes (T2D) and cardiovascular disease (CVD), the effects of hyperglycemia on EVs in humans is unknown. We tested the hypothesis that a 75-g oral glucose tolerance test (OGTT) would promote changes in EVs in relation to CVD risk. Twenty-five obese adults (Age: 52.4 +/- 3.2 year, BMI: 32.5 +/- 1.2 kg/m(2)) were screened for normal glucose tolerance (NGT, n = 8) and prediabetes (PD, n = 17) using American Diabetes Association criteria (75 g OGTT and/or HbA1c). Body composition (bioelectrical impedance) and fitness (VO(2)peak) were measured. Arterial stiffness (augmentation index; AIx) was measured at 0, 60- and 120-min while insulin, glucose, and free fatty acids were evaluated every 30 min during the OGTT to assess CVD risk. Annexin V positive (AV+) and Annexin V negative (AV-) total EVs, platelet EVs (CD31(+)/CD41(+); CD41(+)), leukocyte EVs (CD45(+); CD45(+)/CD41(-)), platelet endothelial cell adhesion molecule (PECAM) (CD31(+)) and endothelial EVs (CD 31(+)/CD41(-); CD105(+)) were collected at 0 and 120 min. There were no differences in age, BMI, or body fat between NGT and PD (all P > 0.63). Total EVs, AV+ CD31(+) (PECAM), and AV+ CD31(+)/CD41(-) (endothelial) EVs decreased after the OGTT (P