Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces
Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces
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DOI：
10.1016/s0021-9673(99)00004-7
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发表时间：
1999-08-06
影响因子：
4.1
通讯作者：
Fréchet, JMJ
中科院分区：
文献类型：
--
作者：
Sykora, D;Svec, F;Fréchet, JMJ
在孔隙源存在的条件下，在50x8 mm的色谱柱范围内，用甲基丙烯酸缩水甘油酯和二甲基丙烯酸乙酯直接自由基共聚制备了多孔整体柱。这些单体的环氧基团通过与二乙胺反应得到了不同程度的修饰，得到了对离子交换色谱有用的1-N， n-二乙胺-2-羟丙基官能团。在对整体进行表征后，在不同流速下对同源的低聚脱氧腺苷酸[pd(a)(12-18)]和低聚胸苷酸[d(pT)(12-24)]进行色谱分离。即使在4 ml/min的高流速下，也能很好地分离寡核苷酸。(C) 1999 Elsevier Science B.V.版权所有
Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)(12-18)] and oligothymidylic acids [d(pT)(12-24)] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. (C) 1999 Elsevier Science B.V. All rights reserved.