CONTRIBUTION OF GRANULOCYTES AND MONOCYTES TO RESISTANCE AGAINST EXPERIMENTAL DISSEMINATED CANDIDA-ALBICANS INFECTION
CONTRIBUTION OF GRANULOCYTES AND MONOCYTES TO RESISTANCE AGAINST EXPERIMENTAL DISSEMINATED CANDIDA-ALBICANS INFECTION
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DOI：
10.1007/bf01975039
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发表时间：
1988-12-01
影响因子：
4.5
通讯作者：
VANFURTH, R
中科院分区：
文献类型：
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作者：
VANTWOUT, JW;LINDE, I;VANFURTH, R
本研究的目的是调查粒细胞和单核细胞对小鼠急性全身念珠菌感染抵抗力的贡献。为此，通过辐射或用环磷酰胺处理诱导粒细胞减少症和单核细胞减少症，并通过用VP-16处理获得单核细胞减少症。 8GY照射小鼠静脉注射1×104白色念珠菌后，感染第1、3、5天，肾脏培养的白色念珠菌数（以CFU/g组织数的几何平均值表示）分别为5.4×104、7.1×106和5.8×107（与正常小鼠相比，p<0.001）。从受辐射动物的肝脏和脾脏中培养的白色念珠菌数量也显着高于正常小鼠（p<0.001）。对于环磷酰胺处理的小鼠，注射1×103白色念珠菌后，肾脏和脾脏中的微生物数量（感染第1天为1.7×104CFU/g，第3天为1.9×106，第5天为3.8×106）和脾脏中微生物数量显着高于正常小鼠（p至少<0.02）。 VP-16诱导的单核细胞减少症不会导致感染后从肾脏或脾脏培养的白色念珠菌数量增加。从这些研究得出的结论是，在全身感染的前五天内，粒细胞而不是单核细胞或渗出巨噬细胞在抵抗白色念珠菌方面发挥着重要作用。
The aim of the present study was to investigate the contribution of granulocytes and monocytes to resistance against an acute systemic candidal infection in mice. To this end granulocytopenia and monocytopenia were induced by irradiation or treatment with cyclophosphamide, and monocytopenia was obtained by treatment with VP-16. After intravenous injection of 1×104Candida albicansinto mice irradiated with 8 GY, the number ofCandida albicanscultured from the kidneys, expressed as the geometric mean of the number of CFU/g tissue, was 5.4×104, 7.1×106and 5.8×107on days 1, 3 and 5 of infection respectively (p<0.001 compared to normal mice). The number ofCandida albicanscultured from the liver and spleen was also significantly higher for irradiated animals than for normal mice (p<0.001). For cyclophosphamide-treated mice the number of organisms in the kidney (1.7×104CFU/g on day 1, 1.9×106on day 3 and 3.8×106on day 5 of infection) and spleen was significantly higher (p at least <0.02) than for nomal mice after injection of 1×103Candida albicans. Monocytopenia induced by VP-16 did not result in an increase in the number ofCandida albicanscultured from the kidney or spleen after infection. From these studies it is concluded that granulocytes and not monocytes or exudate macrophages play an important role in resistance againstCandida albicansduring the first five days of a systemic infection.