Temperature-Sensitive Lipid-Coated Carbon Nanotubes for Synergistic Photothermal Therapy and Gene Therapy
Temperature-Sensitive Lipid-Coated Carbon Nanotubes for Synergistic Photothermal Therapy and Gene Therapy
复制标题
用于协同光热疗法和基因疗法的温度敏感脂质涂层碳纳米管
DOI：
10.1021/acsnano.0c08790
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发表时间：
2021-03-22
期刊：
影响因子：
17.1
通讯作者：
Zhang, Shubiao
中科院分区：
文献类型：
--
作者：
Zhao, Yinan;Zhao, Tianyi;Zhang, Shubiao
光热疗法（PTT）和基因疗法（GT）的组合显示出巨大的潜力，以实现协同抗肿瘤活性。然而，缺乏从载体中控制释放基因仍然是一个严重的障碍。本文采用肽脂质（PL）和蔗糖月桂酸酯（SL）包覆单壁碳纳米管（SCNTs）和多壁碳纳米管（MCNTs），制备了具有良好温度敏感性和光热性能的双功能递送体系（分别表示为SCNT-PS和MCNT-PS）。CNT/siRNA通过沉默生存素表达抑制肿瘤生长，同时在近红外（NIR）光下表现出光热效应。SCNT-PS/siRNA显示出非常高的抗肿瘤活性，导致一些肿瘤的完全抑制。由于PL和SL涂层，由于温度敏感性脂质的相变，它对于全身递送到肿瘤部位和促进siRNA释放是高效的。因此，SCNT-PS/siRNA是一种很有前途的用于组合PTT和GT的抗肿瘤纳米载体。
The combination of photothermal therapy (PTT) and gene therapy (GT) shows great potential to achieve synergistic anti-tumor activity. However, the lack of a controlled release of genes from carriers remains a severe hindrance. Herein, peptide lipid (PL) and sucrose laurate (SL) were used to coat single-walled carbon nanotubes (SCNTs) and multi-walled carbon nanotubes (MCNTs) to form bifunctional delivery systems (denoted SCNT-PS and MCNT-PS, respectively) with excellent temperature-sensitivity and photothermal performance. CNT/siRNA suppressed tumor growth by silencing survivin expression while exhibiting photothermal effects under near-infrared (NIR) light. SCNT-PS/siRNA showed very high anti-tumor activity, resulting in the complete inhibition of some tumors. It was highly efficient for systemic delivery to tumor sites and to facilitate siRNA release owing to the phase transition of the temperature-sensitive lipids, due to PL and SL coating. Thus, SCNT-PS/siRNA is a promising anti-tumor nanocarrier for combined PTT and GT.