Plasma insulin: fluctuations in the "big" insulin component in man after glucose and other stimuli.
Plasma insulin: fluctuations in the "big" insulin component in man after glucose and other stimuli.
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血浆胰岛素：葡萄糖和其他刺激后，人体中“大”胰岛素成分的波动。
DOI：
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发表时间：
1969
影响因子：
15.9
通讯作者：
J. Roth
中科院分区：
文献类型：
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作者：
P. Gorden;J. Roth
血浆经G50葡聚糖凝胶过滤后，通过免疫测定法测定循环胰岛素的两种组分，“小”胰岛素和“大”胰岛素。前一种成分与胰岛素难以区分，而后者更类似于胰岛素原和从胰腺分离的其他胰岛素样物质。在瘦的正常受试者中，血浆胰岛素中大胰岛素的分数（“大”的百分比）。口服葡萄糖后15-30分钟，小于5%;在接下来的一个小时内，大百分比上升2- 8倍，到90-120分钟，占血浆胰岛素的5-29%。在与血浆胰岛素浓度正常相关的特发性葡萄糖不耐受的年轻瘦弱受试者中，观察到了相同的大胰岛素模式。在标准测试期间90-120分钟时大胰岛素百分比升高的瘦受试者中，仅是适度的，在葡萄糖给药前饥饿48小时导致大胰岛素百分比更明显的升高。在对肥胖和肢端肥大症患者给予葡萄糖后的早期，大的百分比高于瘦的患者。升高幅度与空腹血浆胰岛素浓度升高大致相关。到90-120分钟时，肥胖和肢端肥大症患者的大百分比与瘦受试者相同。在少数受试者中，精氨酸和甲苯磺丁脲的静脉注射产生了与口服葡萄糖相似的反应;刺激后早期，大的百分比较低，并随着时间的推移而增加。在两名胰岛细胞癌患者中，big百分比与正常受试者相同。
Plasma was filtered on G50 Sephadex, and two components of circulating insulin, "little" insulin and "big" insulin, were measured by immunoassay. The former component is indistinguishable from insulin, whereas the latter more closely resembles proinsulin and the other insulin-like substances isolated from the pancreas. In thin normal subjects, the fraction of plasma insulin that was big insulin (per cent "big"). 15-30 min after oral glucose, was less than 5%; per cent big rose 2- to 8-fold over the next hour and by 90-120 min represented 5-29% of the plasma insulin. In young thin subjects with idiopathic glucose intolerance associated with normal concentrations of plasma insulin, an identical pattern of big insulin was observed. In thin subjects in whom elevations of per cent big at 90-120 min during the standard test were only modest, starvation for 48 hr before the glucose administration resulted in a more pronounced rise in the per cent big insulin. Early after glucose administration to obese and acromegalic subjects, the per cent big was higher than in thin subjects. The magnitude of the elevation was roughly correlated with elevations in the fasting plasma insulin concentration. By 90-120 min, the per cent big in obese and acromegalic patients was the same range as in the thin subjects. Intravenously administered arginine and tolbutamide in a small number of subjects yielded a response that was similar to oral glucose; the per cent big was low early after stimulation and increased with time. In two patients with islet cell carcinomas, the per cent big was in the same range as in normal subjects.