Signal amplification of glucosamine-6-phosphate based on ribozyme glmS.
Signal amplification of glucosamine-6-phosphate based on ribozyme glmS.
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DOI：
10.1016/j.bios.2014.06.067
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发表时间：
2014-12
影响因子：
12.6
通讯作者：
Yongyun Zhao;Haodong Chen;Feng Du;A. Yasmeen;Juan Dong;Xin Cui;Zhuo Tang
中科院分区：
文献类型：
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作者：
Yongyun Zhao;Haodong Chen;Feng Du;A. Yasmeen;Juan Dong;Xin Cui;Zhuo Tang
建立了一种基于核酶的葡萄糖胺-6-磷酸（GlcN 6P）等温扩增比色检测方法。当与代谢物靶点GlcN 6P结合时，glmS核酶开始自切割以释放RNA片段，该RNA片段可以触发级联信号放大以释放大量的G-四链体DNA酶作为用于比色检测的报告物。鉴于GlcN 6P在细胞壁生物合成中的重要性，glmS核糖开关已成为开发抗生素的新药物靶点。该检测方法不仅具有较高的特异性和灵敏度，而且为基于lmS核糖开关的抗生素高通量筛选提供了一个平台。
RibozymeglmSbased isothermal amplification assay is developed for the colorimetric detection of glucosamine-6-phosphate (GlcN6P). Upon binding to the metabolite target GlcN6P, self-cleavage ofglmSribozyme is initiated to release RNA fragment that can trigger the cascade signal amplification to release large amount of G-quadruplex DNAzymes as reporter for colorimetric detection. Given the importance of GlcN6P for cell wall biosynthesis, theglmSriboswitch has become a new drug target for the development of antibiotics. This assay not only offers a convenient detection of GlcN6P with high specificity and sensitivity, but also provides a platform for high-throughput screening of antibiotics based onglmSriboswitches.