Micro fluid segment technique for screening and development studies on Danio rerio embryos
Micro fluid segment technique for screening and development studies on Danio rerio embryos
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DOI：
10.1039/b701116d
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Koehler, Johann Michael
中科院分区：
文献类型：
--
作者：
Funfak, Anette;Broesing, Andreas;Koehler, Johann Michael
已经研究了微流体片段在研究多细胞系统行为，特别是胚胎发育中的适用性。研究发现，使用全氟甲基十氢化萘（PP9）作为载液，特氟龙（（R））（PTFE）作为管材料，可以将来自斑马鱼斑马鱼的卵引入微流体段中，而不会造成严重损坏。在 80 小时的时间内观察鱼胚胎的发育过程，直至孵化。五天后，将鱼幼体从微流体段中取出并转移到繁殖池中。研究了单独的膜损伤阴离子表面活性剂十二烷基硫酸钠 (SDS) 和添加 CuCl2（氯化铜 (II)）的 SDS 的效果。通过分析不同的终点，我们发现对胚胎发育的抑制和支持作用。含有或不含铜 (II) 离子的低 SDS 浓度具有支持作用，而较高的 SDS 浓度会对胚胎发育产生负面影响。结果表明，可以使用微流体系统进行复杂生物系统的自动化微筛选过程，并且适用于未来的毒理学和药物筛选研究。
The applicability of micro fluid segments for studying the behaviour of multicellular systems, in particular embryonic development, has been investigated. It was found that eggs from the zebrafish Danio rerio can be introduced into micro fluid segments without serious damage by using perfluoromethyldecalin ( PP9) as the carrier liquid and Teflon((R)) ( PTFE) as the tube material. The development processes of fish embryos were observed over a time period of 80 hours, until hatching time. After five days, the fish larvae were brought out of the micro fluid segments and transferred into breeding reservoirs. Effects of the membrane-damaging anionic surfactant sodium dodecyl sulfate ( SDS) alone and SDS with the addition of CuCl2 ( copper( II) chloride) were investigated. By analyzing different end points, we found inhibiting and also supporting effects on the development of the embryos. Low SDS concentrations with and without copper( II) ions were supportive, while higher SDS concentrations led to negative impacts on the development of the embryos. The results showed that automated micro screening processes with complex biological systems can be performed using microfluidic systems and are applicable for future toxicological and drug screening studies.