A novel myosin heavy chain gene in human chromosome 19q13.3
A novel myosin heavy chain gene in human chromosome 19q13.3
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DOI：
10.1016/s0378-1119(03)00613-9
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发表时间：
2003-07-17
期刊：
影响因子：
3.5
通讯作者：
Rautenstrauss, B
中科院分区：
文献类型：
--
作者：
Leal, A;Endele, S;Rautenstrauss, B
通过计算序列分析、RT-PCR和DNA测序，在染色体19 q13上鉴定出一个人肌球蛋白重链基因。该基因全长6786 bp，包含41个外显子（40个编码区），全长108 kb。还鉴定了可变剪接变体。该基因在多种组织中表达，但主要在小肠、结肠和骨骼肌中表达。推定的蛋白质（228 kDa）携带常见的肌球蛋白结构域，并与非肌肉肌球蛋白重链（MYH 9和MYH 10）以及平滑肌肌球蛋白重链MYH 11具有高度同源性。然而，系统发育分析表明，这些同源蛋白之间的关系比MYH 14，这可能是肌球蛋白重链应被归类在一个新的肌球蛋白亚家族。(C)2003 Elsevier Science B. V.保留所有权利。
A human myosin heavy chain gene was identified in chromosome 19q13 by computational sequence analysis, RT-PCR and DNA sequencing of the cDNA. The complete cDNA has a length of 6786 bp and comprises 41 exons (40 coding) included in 108 kb of genomic sequence. Alternative splicing variants were also identified. The gene is expressed in a multitude of tissues, but mainly in small intestine, colon and skeletal muscle. The putative protein (228 kDa) carries the common myosin domains and presents high homology with the nonmuscle myosin heavy chains (MYH9 and MYH10) as well as the smooth muscle myosin heavy chain MYH11. Nevertheless, phylogenetic analysis indicated that these homologous proteins are more related among themselves than to MYH14, suggesting that possibly this myosin heavy chain should be classified in a new myosin-subfamily. (C) 2003 Elsevier Science B.V. All rights reserved.