Standalone cohesin as a molecular shuttle in cellulosome assembly.
Standalone cohesin as a molecular shuttle in cellulosome assembly.
复制标题
独立的粘连蛋白作为纤维素体组装中的分子穿梭机。
DOI：
10.1016/j.febslet.2015.04.013
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发表时间：
2015
期刊：
影响因子：
3.5
通讯作者：
Frolow F.
中科院分区：
文献类型：
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作者：
Voronov-Goldman M;Yaniv O;Gul O;Yoffe H;Salama-Alber O;Slutzki M;Levy-Assaraf M;Jindou S;Shimon LJ;Borovok I;Bayer EA;Lamed R;Frolow F.
食草动物瘤胃的纤维分解细菌黄色瘤胃球菌产生一个精细的纤维素体系统，通过共价结合的支架蛋白SCAE固定在细菌细胞壁上。含有Dockerin的支架还与一种功能未知的自主粘附素结合，称为粘附素G(CohG)。在这里，我们证明了CohG以与SCAE相反的方向结合到支架蛋白携带的Dockerin上。基于这些结构数据，我们认为络合的Dockerin仍然可以与细胞表面的SCAE结合。因此，CohG可以作为一个分子穿梭机将支架运送到细菌细胞表面。
The cellulolytic bacteriumRuminococcus flavefaciensof the herbivore rumen produces an elaborate cellulosome system, anchored to the bacterial cell wall via the covalently bound scaffoldin ScaE. Dockerin‐bearing scaffoldins also bind to an autonomous cohesin of unknown function, called cohesin G (CohG). Here, we demonstrate that CohG binds to the scaffoldin‐borne dockerin in opposite orientation on a distinct site, relative to that of ScaE. Based on these structural data, we propose that the complexed dockerin is still available to bind ScaE on the cell surface. CohG may thus serve as a molecular shuttle for delivery of scaffoldins to the bacterial cell surface.