The alternative splicing of BdTai and its involvement in the development of Bactrocera dorsalis (Hendel)
The alternative splicing of BdTai and its involvement in the development of Bactrocera dorsalis (Hendel)
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BdTai 的选择性剪接及其参与橘小实蝇 (Hendel) 发育的过程
DOI：
10.1016/j.jinsphys.2017.07.012
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发表时间：
2017
影响因子：
2.2
通讯作者：
Dou Wei
中科院分区：
文献类型：
--
作者：
Liu Hong;Li Hui Min;Yue Yong;Song Zhong Hao;Wang Jin Jun;Dou Wei
对昆虫变态的兴趣主要集中在保幼激素 (JH) 和蜕皮激素上。与蜕皮激素信号传导相比，JH 的分子作用尚不明确，因为直到最近才确定了甲氧普林耐受 (Met) 作为 JH 受体。体外研究表明，Met 与一种伙伴蛋白 Taiman (Tai) 形成活性 JH 依赖性复合物。然而，有关Tai在昆虫变态中的作用的相关研究非常有限。在这项研究中，从东方果蝇橘小实蝇（一种重要的世界范围内侵染水果和蔬菜的害虫）中鉴定出 C 端区域不同的五种 Tai 亚型。时空表达模式分析表明，BdTai-A和BdTai-Bad在早期幼虫中表达量较高，而BdTai-Dis在中晚期幼虫中表达量较高。同时，体内甲氧普林应激导致BdTai亚型的表达模式发生剧烈波动。随后的反向遗传学研究表明，所有Tai亚型（称为“Tai-core”）在B.桔小实蝇产生早熟的幼虫-蛹发育，即缩短的化蛹过程和微型蛹。个体Tai亚型的进一步敲低表明BdTai-的沉默导致与BdTai-coreRNAi相同的表型。目前的数据表明，BdTai-E 参与转导抑制变态的 JH 信号。此外，在研究 Tai 函数时应考虑异构体。
Interest in insect metamorphosis has primarily focused on juvenile hormone (JH) and ecdysone. Compared to ecdysone signaling, the molecular action of JH is less well established because Methoprene-tolerant (Met) as the JH receptor has been identified until recently.In vitrostudies have indicated that Met forms an active JH-dependent complex with one partner protein, Taiman (Tai). However, the related studies on Tai’s role in insect metamorphosis are very limited. In this study, five Tai isoforms differing in C-terminal region are identified from the oriental fruit flyBactrocera dorsalis, an important worldwide pest infesting fruits and vegetables. The spatiotemporal expression pattern analysis indicates thatBdTai-AandBdTai-Bare highly expressed in early larvae whileBdTai-Dis more abundant in middle-late larvae. Meanwhile,in vivomethoprene stress leads to dramatic expression pattern fluctuation ofBdTaiisoforms. The subsequent reverse genetic study reveal that all Tai isoforms (denoted as “Tai-core”) depletion in larvae stage ofB. dorsalisproduce precocious larvae-pupae development, i.e. shortened pupation process and miniature pupae. Further knockdown of individual Tai isoform show that silence ofBdTai-Ecauses the same phenotype as ofBdTai-coreRNAi. The current data suggest thatBdTai-Eis involved in transducing the JH signal that represses metamorphosis. Besides, isoforms should be considered when studying Tai functions.