Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3.
Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3.
复制标题
第五种 α2,8-唾液酸转移酶 (ST8Sia V) 的分子克隆和表达。
DOI：
--
复制
发表时间：
1996
影响因子：
4.8
通讯作者：
S. Tsuji
中科院分区：
文献类型：
--
作者：
Mari Kono;Yukiko Yoshida;Naoya Kojima;S. Tsuji
通过基于聚合酶链式反应的方法，使用关于小鼠ST 8 Sia I（GD 3合酶）和小鼠ST 8 Sia III（Siaalpha 2，3Gal β 1，4GlcNAcalalpha 2，8-唾液酸转移酶）的核苷酸序列信息，从小鼠脑cDNA文库中克隆编码新的α 2，8-唾液酸转移酶（ST 8 Sia V）的cDNAs，这两种酶都表现出对糖脂的活性。ST 8 Sia V与小鼠ST 8 Sia I和III的氨基酸序列同源性分别为36.1%和15.0%。在COS-7细胞中表达的重组蛋白A融合的ST 8 Sia V显示出对GM 1b、GD 1a、GT 1b和GD 3的α 2，8-唾液酸转移酶活性，并分别合成GD 1c、GT 1a、GQ 1b和GT3。GM 1b、GD 1a、GT 1b和GD 3的表观Km值分别为1.1、0.082、0.070和0.28 mM。然而，ST 8 Sia V没有表现出对GM 3的活性。因此，ST 8 Sia V的底物特异性不同于ST 8 Sia I和III的底物特异性，两者都表现出对GM 3的活性。将ST 8 Sia V基因转染到COS-7细胞中，其表达GD 1a作为主要糖脂，导致单克隆抗体4F 10的决定簇的表达，其识别GT 1a和GQ 1b，表明ST 8 Sia V在体内对神经节苷脂GD 1a和/或GT 1b表现出活性。ST 8 Sia V基因的表达是组织和发育阶段特异性的，并且与其他α 2，8-唾液酸转移酶基因的表达明显不同。ST 8 Sia V基因在大脑中强烈表达，在其他组织如肝脏中表达较弱。此外，其在成人脑中的表达高于胎脑。这些结果强烈表明，ST 8 Sia V是SAT-V的候选者，SAT-V是参与GD 1c、GT 1a、GQ 1b和GT3合成的α 2，8-唾液酸转移酶。
The cDNAs encoding a new alpha2,8-sialyltransferase (ST8Sia V) were cloned from a mouse brain cDNA library by means of a polymerase chain reaction-based method using the nucleotide sequence information on mouse ST8Sia I (GD3 synthase) and mouse ST8Sia III (Siaalpha2,3Galbeta1,4GlcNAcalpha2,8-sialyltransferase ), both of which exhibit activity toward glycolipids. The predicted amino acid sequence of ST8Sia V shows 36.1% and 15.0% identity to those of mouse ST8Sia I and III, respectively. The recombinant protein A-fused ST8Sia V expressed in COS-7 cells exhibited an alpha2, 8-sialyltransferase activity toward GM1b, GD1a, GT1b, and GD3, and synthesized GD1c, GT1a, GQ1b, and GT3, respectively. The apparent Km values for GM1b, GD1a, GT1b and GD3 were 1.1, 0.082, 0.070, and 0.28 mM, respectively. However, ST8Sia V did not exhibit activity toward GM3. Thus, the substrate specificity of ST8Sia V is different from those of ST8Sia I and III, both of which exhibit activity toward GM3. Transfection of the ST8Sia V gene into COS-7 cells, which express GD1a as a major glycolipid, led to the expression of determinants for monoclonal antibody 4F10, which recognizes GT1a and GQ1b, suggesting that ST8Sia V exhibits activity toward gangliosides GD1a and/or GT1b in vivo. The expression of the ST8Sia V gene was tissue- and developmental stage-specific, and was clearly different from those of other alpha2,8-sialyltransferase genes. The ST8Sia V gene was strongly expressed in the brain and weakly in other tissues such as the liver. In addition, its expression was greater in the adult than fetal brain. These results strongly indicate that ST8Sia V is a candidate for SAT-V, the alpha2,8-sialyltransferase involved in GD1c, GT1a, GQ1b, and GT3 synthesis.
β-半乳糖苷 α 2,6-唾液酸转移酶基因的组织。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Svensson,EC;Soreghan,B;Paulson,JC
通讯作者：
Paulson,JC
DOI：
10.1074/jbc.271.7.3684
发表时间：
1996-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Nakayama;M. Fukuda;Y. Hirabayashi;A. Kanamori;K. Sasaki;T. Nishi;M. Fukuda
通讯作者：
J. Nakayama;M. Fukuda;Y. Hirabayashi;A. Kanamori;K. Sasaki;T. Nishi;M. Fukuda
DOI：
10.1073/pnas.91.22.10455
发表时间：
1994-10-25
影响因子：
11.1
作者：
HARAGUCHI, M;YAMASHIRO, S;FURUKAWA, K
通讯作者：
FURUKAWA, K