Host recognition and integration of filamentous phage φRSM in the phytopathogen, Ralstonia solanacearum
Host recognition and integration of filamentous phage φRSM in the phytopathogen, Ralstonia solanacearum
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DOI：
10.1016/j.virol.2008.11.007
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发表时间：
2009-02-05
期刊：
影响因子：
3.7
通讯作者：
Yamada, Takashi
中科院分区：
文献类型：
--
作者：
Askora, Ahmed;Kawasaki, Takeru;Yamada, Takashi
在青枯菌种复合体的菌株中检测到了两种原噬菌体，称为 phi RSM3 和 phi RSM4，它们与丝状噬菌体 phi RSM1 密切相关但又不同。在宿主菌株MAFF730139中发现的原噬菌体phi RSM3可以通过PCR和转染的方式转化为感染性噬菌体。 phi RSM3 的核苷酸序列相对于 phi RSM7 高度保守，除了编码未知蛋白的开放阅读框 2 (ORF2) 和编码确定宿主范围的假定吸附蛋白的 ORF9 之外。这两个宿主范围差异很大，并且与细胞表面菌毛的不同凝胶电泳条带模式密切相关。 phi RSM1 和 phi RSM3 的感染增强了番茄植株中细菌细胞的聚集并降低了细菌宿主的毒力。对已知基因组序列的青枯菌菌株的数据库搜索发现，在菌株 UW551 的基因组中，有两种丝状病毒原噬菌体，一种称为 phi RSM4，与 phi RSM1 和 phi RSM3 同源，一种与 RSS1 同源。 (C) 2008 Elsevier Inc. 保留所有权利。
Two prophages, called phi RSM3 and phi RSM4, that are closely related to, but differ from, filamentous phage phi RSM1, have been detected in strains of the Ralstonia solanacearum species complex. The prophage phi RSM3, found in host strain MAFF730139, could be converted to infectious phage by means of PCR and transfection. The nucleotide sequence of phi RSM3 is highly conserved relative to phi RSM7 except for open reading frame 2 (ORF2), encoding an unknown protein, and ORF9 encoding the presumed adsorption protein that determines host range. The two host ranges differ dramatically and correlate closely with different gel electrophoresis banding patterns for cell surface fimbriae. Infections by phi RSM1 and phi RSM3 enhance bacterial cell aggregation and reduce the bacterial host virulence in tomato plants. Database searches in the R. solanacearum strains of known genomic sequence revealed two inovirus prophages, one designated phi RSM4 that is homologous to phi RSM1 and phi RSM3, and one homologues to RSS1, in the genome of strain UW551. (C) 2008 Elsevier Inc. All rights reserved.