A comprehensive investigation of the synthesis of prebiotic galactooligosaccharides by whole cells of Bifidobacterium bifidum NCIMB 41171
A comprehensive investigation of the synthesis of prebiotic galactooligosaccharides by whole cells of Bifidobacterium bifidum NCIMB 41171
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DOI：
10.1016/j.jbiotec.2010.08.008
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发表时间：
2010-10-01
影响因子：
4.1
通讯作者：
Charalampopoulos, Dimitris
中科院分区：
文献类型：
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作者：
Osman, Ali;Tzortzis, George;Charalampopoulos, Dimitris
通过建立数学模型，研究了两歧双歧杆菌NCIMB 41171全细胞合成低聚半乳糖（GOS）的过程。这些是二阶多项式方程，其描述了与COS组分的产生、乳糖转化为GOS的选择性以及所产生的GOS混合物的相对组成相关的响应，作为生物催化剂的量、温度、初始乳糖浓度和时间的函数。跟踪合成反应长达36小时。每4小时取样一次，测试β-半乳糖苷酶活性，并分析其碳水化合物含量。GOS的合成均能被模型很好地解释，且均具有显著性差异（P
The synthesis of galactooligosaccharides (GOS) by whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated by developing a set of mathematical models. These were second order polynomial equations, which described responses related to the production of COS constituents, the selectivity of lactose conversion into GOS, and the relative composition of the produced GOS mixture, as a function of the amount of biocatalyst, temperature, initial lactose concentration, and time. The synthesis reactions were followed for up to 36h. Samples were withdrawn every 4h, tested for beta-galactosidase activity, and analysed for their carbohydrate content. GOS synthesis was well explained by the models, which were all significant (P