Reversibility of n-3 fatty acid deficiency-induced changes in dopaminergic neurotransmission in rats: critical role of developmental stage
Reversibility of n-3 fatty acid deficiency-induced changes in dopaminergic neurotransmission in rats: critical role of developmental stage
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DOI：
10.1194/jlr.m200132-jlr200
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发表时间：
2002-08-01
影响因子：
6.5
通讯作者：
Chalon, S
中科院分区：
文献类型：
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作者：
Kodas, E;Vancassel, S;Chalon, S
以往的研究表明，在大鼠慢性氨基酸饮食缺乏的情况下，大脑膜的脂质组成和多巴胺能神经传递发生改变。这项研究调查了这些变化是否可以逆转，以及大脑成熟的阶段是否可能在恢复过程中发挥作用。在已知受这种缺乏影响的脑区域（即，前额叶皮层和丘脑核）。用双探针微透析法研究了药物刺激下多巴胺的释放。囊泡单胺转运蛋白进行了研究，采用定量放射自显影。逆转饮食，与足够的n-6和n-3多不饱和脂肪酸（PUFA）的水平，给予缺乏大鼠在不同的发展阶段（0，7，14，或21天的年龄）。结果表明，当在哺乳期，这种饮食是能够恢复的FA组成的大脑膜和多巴胺能神经传递的参数的研究。然而，当断奶后给予时，它可以部分恢复生化参数，但不能恢复神经化学因子。(jlr)因此，在哺乳期发生严重的n-3 PUFA缺乏可能是一种环境损害，导致对特定脑功能的不可逆损害。
Previous investigations have shown that the lipid composition of cerebral membranes and dopaminergic neurotransmission are changed under chronic aminolenic acid diet deficiency in the rat. This study investigated whether these changes could be reversed and if the stage of brain maturation might play a role in the recovery process. The effects of reversion on the fatty acid (FA) composition and dopaminergic neurotransmission were studied in brain regions known to be affected by such deficiency (i.e., the prefrontal cortex and nucleus accumbens) in 2-month-old animals. Dopamine release under pharmacological stimulation was studied using a dual-probe microdialysis method. Vesicular monoamine transporters were studied using quantitative autoradiography. The reversal diet, with adequate levels of n-6 and n-3 polyunsaturated fatty acids (PUFAs), was given to deficient rats at different stages of development (0, 7, 14, or 21 days of age). The results showed that when given during the lactating period, this diet was able to restore both the FA composition of brain membranes and the parameters of dopaminergic neurotransmission studied. However, when given from weaning, it allowed partial recovery of biochemical parameters but no recovery of neurochemical factors.(jlr) The occurrence of profound n-3 PUFA deficiency during the lactating period could therefore be an environmental insult leading to irreversible damage to specific brain functions.