Evaluation of factors promoting astaxanthin production by a unicellular green alga, Haematococcus pluvialis, with fractional factorial design
Evaluation of factors promoting astaxanthin production by a unicellular green alga, Haematococcus pluvialis, with fractional factorial design
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DOI：
10.1021/bp025549b
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发表时间：
2002-11-01
影响因子：
2.9
通讯作者：
Park, JM
中科院分区：
文献类型：
--
作者：
Choi, YE;Yun, YS;Park, JM
采用序贯部分析因设计对单细胞绿色球藻雨生红球藻UTEX 16产虾青素的影响因素进行了研究。为了模拟实际生产模式，采用两阶段工艺生产虾青素：首先在营养生长条件下培养酵母，然后通过应用各种诱导方法诱导虾青素生产。高剂量的辐射是最有效的生产虾青素的重量（毫克/克）和细胞（pg/细胞）的内容。氮缺乏和乙酸盐添加的组合也显着增加了细胞的虾青素含量的干重的基础上。同时，单独添加乙酸盐仅增加细胞内的虾青素含量。虽然单独添加亚铁离子对虾青素的重量含量有负面影响，但同时添加亚铁离子和乙酸盐可有效增加虾青素的细胞含量。
Factors affecting the astaxanthin production by a unicellular green alga, Haematococcus pluvialis UTEX 16, were evaluated with sequential fractional factorial design. To simulate an actual production mode, a two-stage process was adapted for astaxanthin production: the alga was first cultivated under vegetative growth conditions, and then astaxanthin production was induced by applying various induction methods. A high dose of irradiation was most effective for the production of astaxanthin both in weight (mg/g) and in cellular (pg/cell) contents. A combination of nitrogen deficiency and acetate addition also significantly increased the astaxanthin content of cells on a dry weight basis. Meanwhile, the acetate addition alone increased only the cellular content of astaxanthin. Although the addition of ferrous ion alone had a negative effect on the weight content of astaxanthin, simultaneous addition of ferrous ion and acetate was effective for increasing the cellular content of astaxanthin.