Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast
Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast
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DOI：
10.1016/s1097-2765(00)80324-x
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发表时间：
2000-05-01
期刊：
影响因子：
16
通讯作者：
Snyder, M
中科院分区：
文献类型：
--
作者：
Barral, Y;Mermall, V;Snyder, M
特殊质膜结构域的形成和维持对于许多生物过程至关重要，例如细胞极化和信号传导。在各向同性芽生长过程中，酵母细胞外围分为两个区域：芽表面（胞吐作用和生长的活性位点）和母细胞相对静止的表面。我们发现，在各向同性生长过程中，芽颈处缺乏脓蛋白的细胞无法维持芽中的胞吐作用和形态发生因子 Spa2、Sec3、Sec5 和 Myo2。此外，我们发现脓毒蛋白对于肌动蛋白斑块稳定性的适当调节是必需的。脓蛋白缺陷细胞允许进入各向同性生长，失去肌动蛋白和生长极性。我们提出脓毒症通过指定皮质域之间的边界来维持细胞极性。
Formation and maintenance of specialized plasma membrane domains are crucial for many biological processes, such as cell polarization and signaling. During isotropic bud growth, the yeast cell periphery is divided into two domains: the bud surface, an active site of exocytosis and growth, and the relatively quiescent surface of the mother cell. We found that cells lacking septins at the bud neck failed to maintain the exocytosis and morphogenesis factors Spa2, Sec3, Sec5, and Myo2 in the bud during isotropic growth. Furthermore, we found that septins were required for proper regulation of actin patch stability; septin-defective cells permitted to enter isotropic growth lost actin and growth polarity. We propose that septins maintain cell polarity by specifying a boundary between cortical domains.