A biphasic pattern of gene expression during mouse retina development.
A biphasic pattern of gene expression during mouse retina development.
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DOI：
10.1186/1471-213x-6-48
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发表时间：
2006-10-17
影响因子：
--
通讯作者：
Fu XY
中科院分区：
文献类型：
--
作者：
Zhang SS;Xu X;Liu MG;Zhao H;Soares MB;Barnstable CJ;Fu XY
胚胎第12天和出生后第21天之间，六个主要的神经元和一个神经胶质细胞类型产生的多能祖细胞在一个特征性的序列在小鼠视网膜发育。为了系统研究小鼠视网膜发育过程中主要胚胎期和出生后发育阶段视网膜转录本的表达模式，我们从小鼠视网膜中收集了一组非冗余EST序列克隆，构建了一个组织特异性cDNA微阵列。收集从胚胎第12.5天（E12.5）到出生后第21天（PN 21）的11个阶段的小鼠视网膜用于RNA分离。标记未扩增的RNA用于微阵列实验，并分析三组数据的显著性、层次关系和功能聚类。通过视网膜发育的转录本的表达模式的基础上，确定了六个单独的基因表达簇。两个发育阶段，明确划分与出生后第5天（PN 5）作为一个单独的集群。在发育过程中发生显著变化的4，180个转录物中，约2/3的基因在PN 5之前以高水平表达，然后下降，而其他1/3的基因从PN 5开始增加表达，并保持在较高水平，直到至少PN 21。不到1%的基因在两个阶段之间出现表达高峰。在后来增加的群体中，只有大约40%的基因与视杆细胞相关，表明在这个阶段多种细胞类型有助于基因表达。然而，在相同的功能类，不同的基因群体在不同的发育阶段表达。相关系数分析的基因表达在视网膜发育过程中以前的SAGE研究和本研究之间也进行了。这项研究提供了一个互补的基因组范围内的共同基因动态和广泛的小鼠视网膜发育的分子分类。同一功能簇中的不同基因在不同发育阶段表达，表明细胞从分化到成熟阶段可能改变基因表达谱。我们认为，在发育过程中基因调控的大规模变化是视网膜最终成熟和功能所必需的。
Between embryonic day 12 and postnatal day 21, six major neuronal and one glia cell type are generated from multipotential progenitors in a characteristic sequence during mouse retina development. We investigated expression patterns of retina transcripts during the major embryonic and postnatal developmental stages to provide a systematic view of normal mouse retina development, A tissue-specific cDNA microarray was generated using a set of sequence non-redundant EST clones collected from mouse retina. Eleven stages of mouse retina, from embryonic day 12.5 (El2.5) to postnatal day 21 (PN21), were collected for RNA isolation. Non-amplified RNAs were labeled for microarray experiments and three sets of data were analyzed for significance, hierarchical relationships, and functional clustering. Six individual gene expression clusters were identified based on expression patterns of transcripts through retina development. Two developmental phases were clearly divided with postnatal day 5 (PN5) as a separate cluster. Among 4,180 transcripts that changed significantly during development, approximately 2/3 of the genes were expressed at high levels up until PN5 and then declined whereas the other 1/3 of the genes increased expression from PN5 and remained at the higher levels until at least PN21. Less than 1% of the genes observed showed a peak of expression between the two phases. Among the later increased population, only about 40% genes are correlated with rod photoreceptors, indicating that multiple cell types contributed to gene expression in this phase. Within the same functional classes, however, different gene populations were expressed in distinct developmental phases. A correlation coefficient analysis of gene expression during retina development between previous SAGE studies and this study was also carried out. This study provides a complementary genome-wide view of common gene dynamics and a broad molecular classification of mouse retina development. Different genes in the same functional clusters are expressed in the different developmental stages, suggesting that cells might change gene expression profiles from differentiation to maturation stages. We propose that large-scale changes in gene regulation during development are necessary for the final maturation and function of the retina.