Crypthecodinium cohnii and Schizochytrium sp as potential substitutes to fisheries-derived oils from seabream (Sparus aurata) microdiets
Crypthecodinium cohnii and Schizochytrium sp as potential substitutes to fisheries-derived oils from seabream (Sparus aurata) microdiets
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DOI：
10.1016/j.aquaculture.2008.02.005
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发表时间：
2008-05-12
期刊：
影响因子：
4.5
通讯作者：
Izquierdo, M. S.
中科院分区：
文献类型：
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作者：
Ganuza, E.;Benitez-Santana, T.;Izquierdo, M. S.
生产了两种单细胞异养生物作为二十二碳六烯酸（DHA；22:6 n-3）的渔业来源油的替代来源。在金头鲷 (Sparus aurata) 微量饲料中测试均质化（分别为 S-H、C-H）或非均质化（S、C）的裂殖壶菌 G13/2S 或 Cypthecodinium cohnii 生物质。在试验1中，两种藻类的生物质都用于替代鱼油，而在试验2中，所有膳食脂质，包括源自鱿鱼粉的脂质，在除对照膳食之外的所有膳食中都被藻类生物质所取代。当仅用鱼油替代时，幼鱼的饮食接受度、总体存活率、空气暴露应激存活率、生长和抗病性并未受到所用 DHA 来源的显着影响。然而，用藻类生物质完全替代所有膳食脂质会导致生长和存活率下降，这显然与除了 DHA 含量之外的其他膳食失衡有关，例如 DHA:EPA（二十碳五烯酸；20:5 n-3）比例的变化。在试验 1（0.1 mg 初始幼虫干重）和试验 2（0.15 mg 初始幼虫干重）中分别用实验饲料饲喂 S. aurata 幼虫 15 和 21 天后，藻类生物量包含或均质化并没有显着影响鱼体内的 DHA 沉积（14.4 +/- 0.3% 总脂肪酸 SEM 和 28.4 +/- 0.4，平均值为分别为试验 1 和 2）。在喂食裂殖壶菌属的幼虫中发现较高的花生四烯酸水平。试验 2 中的饮食，并且与二十二碳五烯酸（DPA；22:5 n-6）的逆向转化有关，二十二碳五烯酸在这些饮食中含量很高。这些结果表明单细胞异养生物作为金头鲷微饲料中鱼油的替代 DHA 来源的潜力，但也指出 EPA 来源完全取代渔业来源的油的必要性。 (C) 2008 Elsevier B.V. 保留所有权利。
Two single cell heterotrophs were produced as alternative sources of docosahexaenoic acid (DHA; 22:6 n-3) to fisheries-derived oils. Schizochytrium G13/2S or Cypthecodinium cohnii biomasses, either homogenised (S-H, C-H, respectively) or non-homogenised (S, C), were tested in gilthead seabream (Sparus aurata) microdiets. In Trial 1 the biomases of both species algae were used in replacement of fish oil, whereas in Trial 2 all dietary lipids, including those derived from squid meal, were replaced by the algal biomass in all diets except for control diet. Larval dietary acceptance, overall survival, air-exposure stress survival, growth and disease resistance were not significantly affected by the source of DHA employed when only fish oil was substituted. However, complete substitution of all dietary lipids by the algal biomass, resulted in reduced growth and survival which was apparently related to other dietary imbalances besides simply DHA content, such as changes in the DHA:EPA (eicosapentaenoic acid; 20:5 n-3) ratio. After 15 and 21 d feeding the experimental diets to S. aurata larvae in Trial 1 (0.1 mg initial larval dry weight) and Trial 2 (0.15 mg initial larval dry weight), respectively, the algal biomass inclusion or homogenisation did not significantly affect the DHA deposition in fish body (14.4 +/- 0.3% total fatty acids SEM and 28.4 +/- 0.4, average values for Trials 1 and 2, respectively). Higher arachidonic acid levels were found in larvae fed Schizochytrium sp. diets in Trial 2 and were related to the retro-conversion from docosapentaenoic acid (DPA; 22:5 n-6) which was high in these diets. These results show the potential of single cell heterotrophs as alternative DHA sources for fish oil in microdiets for gilthead seabream but also point out the necessity of EPA sources to completely replace fisheries-derived oils. (C) 2008 Elsevier B.V. All rights reserved.