ORIGIN OF THE INNER CELL MASS IN MOUSE EMBRYOS - CELL LINEAGE ANALYSIS BY MICROINJECTION
ORIGIN OF THE INNER CELL MASS IN MOUSE EMBRYOS - CELL LINEAGE ANALYSIS BY MICROINJECTION
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DOI：
10.1016/0012-1606(86)90327-1
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发表时间：
1986-10-01
影响因子：
2.7
通讯作者：
BALAKIER, H
中科院分区：
文献类型：
--
作者：
PEDERSEN, RA;WU, K;BALAKIER, H
小鼠内细胞群是在8细胞期后被分配到内部位置的细胞形成的。我们通过将两种细胞谱系标记物——辣根过氧化物酶和罗丹明缀合的葡聚糖微注射到8- 32个细胞阶段的小鼠胚泡中，分析了这种分配的时机。前瞻性分析通过联合注射过氧化物酶和葡聚糖，然后进行12-22小时的培养和过氧化物酶活性染色；回顾性分析通过单独注射过氧化物酶和姐妹细胞定位而不进一步培养。两种方法都表明，细胞在小鼠胚胎的第四和第五次卵裂分裂时被分配到内部位置，而不是在第六次卵裂分裂时。因此，在桑葚胚晚期/囊胚早期（32个细胞）阶段，外部细胞可以有内部后代，但在第五次卵裂分裂后留在外部的细胞仅限于滋养外胚层的命运。这些关于细胞谱系的信息表明，先前观察到的哺乳动物胚胎细胞切割的全能性是正常发育中使用的调节属性。
The mouse inner cell mass is established by cells that are allocated to internal positions after the 8-cell stage. We analyzed the timing of this allocation by microinjecting two cell lineage markers, horseradish peroxidase and rhodamineconjugated dextran, into mouse blastomers at the 8- to 32-cell stage. Prospective analysis was performed by coinjection of peroxidase and dextran, followed by 12-22 hr of culture and staining for peroxidase activity; retrospective analysis was performed by injection of peroxidase alone and localization of sister cells without further culture. Both approaches indicated that cells are allocated to internal positions during the fourth and fifth cleavage divisions, but not the sixth cleavage division, of the mouse embryo. Thus, outer cells can have inner descendants until the late morula/early blastocyst (32-cell) stage, but cells remaining outside after the fifth cleavage division are restricted to a trophectoderm fate. This information about cell lineage indicates that the previously observed totipotency of the cleaving mammalian embryo''s cells is a regulative attribute that is used in normal development.