The S-oxalin, N-acetyl-S-oxalylcysteamine, inhibits lymphocyte proliferation, IL-2 production and utilization.
The S-oxalin, N-acetyl-S-oxalylcysteamine, inhibits lymphocyte proliferation, IL-2 production and utilization.
复制标题
S-oxalin（N-乙酰基-S-草酰半胱胺）抑制淋巴细胞增殖、IL-2 的产生和利用。
DOI：
10.1006/bbrc.1996.0774
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Mastro,AM
中科院分区：
文献类型：
--
作者：
Grove,DS;Crowl,CV;Hamilton,GA;Mastro,AM
S-草酸是最近描述的一类细胞代谢物，其似乎作为增殖的负调节剂起作用。以前我们已经证明外源性S-草酰谷胱甘肽（GS-Ox）通过抑制IL-2的产生和利用来抑制淋巴细胞的增殖。在本研究中，在类似的实验中使用合成的S-Oxalin（N-乙酰基-S-草酰半胱胺（ACS-Ox））来确定GS-Ox本身或γ-谷氨酰转移酶（GGT）初始转化GS-Ox后形成的可能的某种代谢产物是否对该效应负责（ACS-Ox不被GGT代谢）。ACS-Ox抑制伴刀豆球蛋白A刺激的淋巴细胞中的DNA合成类似于GS-Ox。IL-2的产生和利用以及IL-2 R的表达也受到抑制。在与GS-Ox相同的浓度下，ACS-Ox也抑制IL-2依赖性细胞的增殖。由于GS-Ox和ACS-Ox的作用非常相似，因此推测S-草酸本身而不是某些代谢物是观察到的作用的原因。草酰基从S-甜菜碱转移到各种蛋白质硫醇是最可能的机制。
S-Oxalins are a recently described class of cell metabolites that appear to function as negative regulators of proliferation. Previously we have shown that exogenous S-oxalylglutathione (GS-Ox) inhibits the proliferation of lymphocytes by inhibiting the production and utilization of IL-2. In the present study the synthetic S-oxalin, N-acetyl-S-oxalylcysteamine (ACS-Ox), was utilized in similar experiments to determine whether GS-Ox itself, or possibly some metabolite formed following initial conversion of GS-Ox by γ-glutamyltransferase (GGT), is responsible for the effects (ACS-Ox is not metabolized by GGT). ACS-Ox inhibited DNA synthesis in lymphocytes stimulated by concanavalin A similarly to GS-Ox. IL-2 production and utilization and IL-2R expression were inhibited as well. ACS-Ox also inhibited the proliferation of IL-2 dependent cells at the same concentration as GS-Ox. Because the effects of GS-Ox and ACS-Ox are so similar, presumably the S-oxalin itself, rather than some metabolite, is responsible for the observed effects. Transfer of oxalyl groups from S-oxalins to various protein thiols is the most likely mechanism involved.