Site-selective proteolytic cleavage of plant viruses by photoactive chiral nanoparticles
Site-selective proteolytic cleavage of plant viruses by photoactive chiral nanoparticles
复制标题
光活性手性纳米粒子对植物病毒的定点蛋白水解切割
DOI：
10.1038/s41929-022-00823-1
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发表时间：
2022-08-01
期刊：
影响因子：
37.8
通讯作者：
Kuang, Hua
中科院分区：
文献类型：
--
作者：
Gao, Rui;Xu, Liguang;Kuang, Hua
植物的病毒病与巨大的健康和经济成本有关。为哺乳动物生物体开发的抗病毒药物对植物的成功有限，因此有必要采取替代战略来解决这一生物和可持续性问题。在这里，我们展示了手性的3 nm Cu1.96S纳米粒子可以在阳光下选择性地裂解烟草花叶病毒的衣壳。以D-青霉胺为表面配体，纳米粒通过超分子键网络与衣壳中的Gln 99至Ala 105片段显示出高的亲和力，而与其他病毒的衣壳的亲和力则低3,000-10,000倍。绿光照射会导致ASN 101和Pro 102之间的酰胺键发生偏振依赖的、蛋白水解酶样的水解。在避免过敏反应和较大的环境影响的同时，纳米粒子在原生质体和植物中分别抑制了98.7%和92.6%的病毒侵染性。这些发现表明，纳米粒子结合了金属离子的蛋白分解活性和纳米手性的位置选择性，可以作为有效的抗病毒药物。
Viral diseases of plants are associated with large health and economic costs. Antiviral agents developed for mammalian organisms have had limited success for plants, necessitating alternative strategies to address this biological and sustainability problem. Here we show that chiral 3 nm Cu1.96S nanoparticles can site-selectively cleave capsid in tobacco mosaic virus under sunlight. With D-penicillamine as surface ligands, the nanoparticles display high affinity to the Gln 99 to Ala 105 segment in the capsid via a network of supramolecular bonds and 3,000-10,000 times lower affinity to capsids of other viruses. Illumination with green light leads to polarization-dependent, protease-like hydrolysis of the amide bond between Asn 101 and Pro 102. Nanoparticles inhibited viral infectivity by 98.7% in protoplasts and 92.6% in plants while avoiding hypersensitive response and large environmental impact. These findings show that nanoparticles combining proteolytic activity due to metal ions and site selectivity due to nanoscale chirality can be used as effective antiviral agents.