hE-cadherin-Fc fusion protein coated surface enhances the adhesion and proliferation of human mesenchymal stem cells.
hE-cadherin-Fc fusion protein coated surface enhances the adhesion and proliferation of human mesenchymal stem cells.
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DOI：
10.1016/j.colsurfb.2013.03.042
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发表时间：
2013-09
期刊：
影响因子：
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通讯作者：
Jianbin Xu;Chuanshun Zhu;Yan Zhang;Nan Jiang;Suhua Li;Zhongyu Su;T. Akaike;Jun Yang
中科院分区：
文献类型：
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作者：
Jianbin Xu;Chuanshun Zhu;Yan Zhang;Nan Jiang;Suhua Li;Zhongyu Su;T. Akaike;Jun Yang
A fusion protein consisting of human E-cadherin extracellular domain and the immunoglobulin G Fc region (hE-cadherin–Fc) was prepared and used as a cell–cell adhesion biomimicking matrix for the in vitro expansion of human mesenchymal stem cells (hMSCs) for use in regenerative medicine. The hE-cadherin–Fc was stably immobilized onto a polystyrene plate due to the hydrophobicity of the Fc domain, enhancing the surface wettability and topography of the plate. The hE-cadherin–Fc matrix markedly promoted the cell adhesion and proliferation of hMSCs compared with the tissue culture-treated plate (TC-PS) and the gelatin-coated plate. Furthermore, the expanded hMSCs on the hE-cadherin–Fc were positive for CD105, similar to those from the gelatin. Additionally, the expression of E-cadherin and β-catenin in the hMSCs was improved on the hE-cadherin–Fc matrix, suggesting that the interactions of the hE-cadherin–Fc matrix with the hMSCs were substitutes for the cell–cell adhesion junctions during the initial culture stage in the absence of intercellular interactions. The hE-cadherin–Fc was shown to be a promising artificial ECM for the in vitro expansion of hMSCs.
