Expression of the homophilic adhesion molecule, Ep-CAM, in the mammalian germ line
Expression of the homophilic adhesion molecule, Ep-CAM, in the mammalian germ line
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DOI：
10.1530/jrf.0.1160379
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发表时间：
1999-07-01
期刊：
影响因子：
--
通讯作者：
Wylie, C
中科院分区：
文献类型：
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作者：
Anderson, R;Schaible, K;Wylie, C
在正常胚胎发育过程中，哺乳动物生殖细胞通过细胞迁移和聚集形成原始性索。生殖细胞必须能够与它们的环境和彼此相互作用才能实现这一目标；然而，早期胚鳝黏附的分子基础尚不清楚。由于生殖细胞在分化过程中会从外周向管腔移动，因此在成人精小管中也会发生差异粘附。在筛选由种系表达的其他粘附分子时，利用免疫细胞化学和带有Ep-CAM特异性单克隆抗体的流式细胞术，在胚胎、新生儿和成人生殖细胞中发现了亲同性粘附分子Ep-CAM的表达。在胚胎期，生殖细胞在胚胎第10.5天的迁移过程中表达Ep-CAM，在胚胎第12.5天的早期性腺组装过程中表达Ep-CAM。Ep-CAM在新生儿雄性和雌性生殖细胞中均有表达。在成年睾丸中，Ep-CAM仅在精原细胞中检测到，在分化程度较高的细胞中不存在。最后，胚胎干细胞被证明表达这种受体。提示Ep-CAM在种系发育和全能性细胞行为中起一定作用。
During normal embryonic development, mammalian germ cells use both cell migration and aggregation to form the primitive sex cords. Germ cells must be able to interact with their environment and each other to accomplish this; however, the molecular basis of early germ eel adhesion is not well characterized. Differential adhesion is also thought to occur in the adult seminiferous tubules, since germ cells move from the periphery to the lumen as they differentiate. In a screen for additional adhesion molecules expressed by the germ line, expression of the homophilic adhesion molecule, Ep-CAM, was identified in embryonic, neonatal and adult germ cells using immunocytochemistry and flow cytometry with an Ep-CAM-specific monoclonal antibody. At embryonic stages, germ cells were found to express Ep-CAM during migration at embryonic day 10.5 and early gonad assembly at embryonic day 12.5. Expression of Ep-CAM was also found on neonatal male and female germ cells. Ln the adult testis, Ep-CAM was detected only on spermatogonia, and was absent from more differentiated cells. Finally, embryonic stem cells were shown to express this receptor. It is proposed that Ep-CAM plays a role in the development of the germ line and the behaviour of totipotent cells.