Comparative study of human aortic and mitral valve interstitial cell gene expression and cellular function.
Comparative study of human aortic and mitral valve interstitial cell gene expression and cellular function.
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DOI：
10.1016/j.ygeno.2013.03.004
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发表时间：
2013-06
期刊：
影响因子：
4.4
通讯作者：
Wei Sun;Rong Zhao;Yang Yang-Yang;Hui Wang;Y. Shao;X. Kong
中科院分区：
文献类型：
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作者：
Wei Sun;Rong Zhao;Yang Yang-Yang;Hui Wang;Y. Shao;X. Kong
瓣膜间质细胞（VICs）在瓣膜发病中起着至关重要的作用。然而，人类主动脉静脉血栓（havic）和二尖瓣静脉血栓（hmvic）的转录谱和细胞功能尚未被直接比较。我们对hAVICs和hmvic进行了NimbleGen基因表达谱分析。已知的78个基因在havic和hmvic之间存在差异表达。与hmvic相比，haic中NKX2-5、TBX15、OGN、OMD和CDKN1C的表达较高，TBX5、MMP1和PCDH10的表达较低。除OGN和OMD外，这些基因的差异在风湿性vic中仍然存在。我们还比较了细胞的增殖、迁移和对矿化介质的反应。在矿化培养基中培养后，hmvic比havic增殖更快，但钙沉积和碱性磷酸酶活性更高，表明hmvic更容易发生体外钙化。我们的研究结果揭示了hAVICs和hmvic在转录谱和细胞功能上的差异。
Valve interstitial cells (VICs) are essential for valvular pathogenesis. However, the transcriptional profiles and cellular functions of human aortic VICs (hAVICs) and mitral VICs (hMVICs) have not been directly compared. We performed NimbleGen gene expression profiling analyses of hAVICs and hMVICs. Seventy-eight known genes were differentially expressed between hAVICs and hMVICs. Higher expression of NKX2-5, TBX15, OGN, OMD, and CDKN1C and lower expression of TBX5, MMP1, and PCDH10 were found in hAVICs compared to hMVICs. The differences in these genes, excepting OGN and OMD, remained in rheumatic VICs. We also compared cell proliferation, migration, and response to mineralization medium. hMVICs proliferated more quickly but showed more calcium deposition and alkaline phosphatase activity than hAVICs after culture in mineralization medium, indicating that hMVICs were more susceptible to in vitro calcification. Our findings reveal differences in the transcription profiles and cellular functions of hAVICs and hMVICs.