Immunohistochemical analysis on aquaporin-1 and aquaporin-3 in skin wounds from the aspects of wound age determination
Immunohistochemical analysis on aquaporin-1 and aquaporin-3 in skin wounds from the aspects of wound age determination
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DOI：
10.1007/s00414-017-1725-0
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发表时间：
2018-01-01
影响因子：
2.1
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
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作者：
Ishida, Yuko;Kuninaka, Yumi;Kondo, Toshikazu
应用免疫组织化学方法对法医学尸体解剖中人皮肤创面水通道蛋白（AQP）1和AQP 3的表达进行了研究。共收集了55个不同施伤后间隔的人皮肤伤口，如下：组I，0-3天（n = 16）;组II，4-7天（n = 11）;组III，9-14天（n = 16）;组IV，17-21天（n = 12）。在未受伤的皮肤样品中，AQP 1和AQP 3可以轻微检测到真皮血管和角质形成细胞，分别。AQP 1（+）血管百分比和AQP 3（+）角质形成细胞数随创面愈合时间延长而明显增加。AQP 3（+）角质形成细胞的数量在组II和组III中明显。与其他3组相比，Ⅱ组AQP 1（+）血管面积和AQP 3（+）细胞数均显著增加。关于法医安全性，AQP 1（+）血管面积超过5%意味着伤口年龄为4-12天。此外，> 15%的阳性面积表明伤口年龄为7-10天。特别地，除了仅一个样品（10天的伤口）外，5-10天的皮肤伤口的大多数样品显示AQP 3（+）细胞数> 300，其余样品具有< 300的细胞数。因此，> 300的AQP 3（+）细胞数将指示5-10天的伤口年龄。总的来说，AQP 1和AQP 3在人类皮肤伤口的免疫组化分析将支持伤口年龄确定的客观准确性。
Immunohistochemical investigation of aquaporin (AQP)1 and AQP3 was performed in human skin wounds obtained from forensic autopsy cases. A total of 55 human skin wounds of different postinfliction intervals were collected as follows: group I, 0-3 days (n = 16); II, 4-7 days (n = 11); III, 9-14 days (n = 16); and IV, 17-21 days (n = 12). In uninjured skin samples, AQP1 and AQP3 could be slightly detected in dermal vessels and keratinocytes, respectively. The percentage of AQP1(+) vessels and the number of AQP3(+) keratinocytes were apparently elevated in accordance with wound ages. The number of AQP3(+) keratinocytes was distinctly evident in groups II and III. Morphometrically, both AQP1(+) vessel area and AQP3(+) cell number were markedly increased in group II, compared with other three groups. With regard to forensic safety, AQP1(+) vessel area of over 5% would imply wound ages of 4-12 days. Moreover, the positive area of > 15% would suggest wound age of 7-10 days. Especially, most samples of skin wounds aged 5-10 days except for only one sample (a 10-day-old wound) showed AQP3(+) cell number of > 300, and the remaining other samples had that of < 300. Thus, the AQP3(+) cell number of > 300 would indicate wound ages of 5-10 days. Collectively, immunohistochemical analyses of AQP1 and AQP3 in human skin wounds would support the objective accuracy of wound age determination.