Characterization of a monoclonal antibody that recognizes a lymphocyte surface antigen for the cetacean homologue to CD45R
Characterization of a monoclonal antibody that recognizes a lymphocyte surface antigen for the cetacean homologue to CD45R
复制标题
识别 CD45R 鲸类同源物的淋巴细胞表面抗原的单克隆抗体的表征
作者：
S. De Guise;K. Erickson;M. Blanchard;L. Dimolfetto;H. Lepper;J. Wang;J. Stott;D. Ferrick
作为我们目前努力开发的测定和试剂，以研究海洋哺乳动物的免疫系统的一部分，并鉴于目前所作的努力，以开发单克隆抗体的细胞表面蛋白的淋巴细胞亚群在不同的物种，本文件报告的表征单克隆抗体对同源的CD45受体的鲸类动物淋巴细胞。该抗体的特异性已根据其识别抗原的免疫沉淀、鲸类淋巴结和胸腺切片的免疫过氧化物酶染色以及鲸类外周血单核细胞和胸腺、淋巴结和脾脏单细胞悬液的单色和双色流式细胞术分析进行表征。抗鲸目动物CD45 R（F21.H）免疫沉淀蛋白质分子量为180、200和220×103 MW，其中180×103 MW形式主要在T细胞上表达，220×103 MW形式主要在B细胞和胸腺细胞上表达。F21.H标记了脾细胞单细胞悬液上的所有B细胞和部分T细胞。CD45R−杀人鲸外周血淋巴细胞表达的CD2密度高于CD45R+，这是记忆T细胞的特征。刀豆球蛋白A（Con A）和植物血凝素（PHA）激活后，虎鲸T淋巴细胞也失去了CD45 R的表达。这是鲸目动物中CD45 R单克隆抗体的第一份报告，这种抗体被预见为一种可能有价值的诊断和研究工具，以评估圈养和野生鲸目动物的免疫功能，作为其健康状况评估的一部分。
As part of our current efforts to develop assays and reagents to study the immune system of marine mammals, and in view of the effort currently made to develop monoclonal antibodies to cell surface proteins of lymphocyte subsets in different species, the present paper reports on the characterization of a monoclonal antibody against the homologue of CD45R on cetacean lymphocytes. The specificity of this antibody has been characterized on the basis of immunoprecipitation of the antigen it recognized, immunoperoxidase staining on cetacean lymph node and thymus sections, as well as one and two‐colour flow cytometric analysis of cetacean peripheral blood mononuclear cells and single‐cell suspensions of thymus, lymph node and spleen. Anti‐cetacean CD45R (F21.H) immunoprecipitated proteins of 180, 200 and 220×103 MW, with the 180×103 MW form being predominantly expressed on T cells and the 220×103 MW form expressed predominantly on B cells and thymocytes. F21.H labelled all B cells and a proportion of T cells on single‐cell suspensions of spleen cells. CD45R− killer whale peripheral blood lymphocytes expressed a higher density of CD2 than CD45R+, a characteristic of memory T cells. Killer whale T lymphocytes also lost the expression of CD45R upon activation with concanavalin A (Con A) and phytohaemagglutinin (PHA). This is the first report of a monoclonal antibody to CD45R in cetaceans, and this antibody is foreseen as a possible valuable diagnostic and research tool to assess immune functions of captive and wild cetaceans as part of the evaluation of their health status.
证明白细胞共同抗原CD45是一种蛋白酪氨酸磷酸酶。
影响因子：
2.9
作者：
Tonks,NK;Charbonneau,H;Diltz,CD;Fischer,EH;Walsh,KA
通讯作者：
Walsh,KA
人胸腺细胞对 CD2 抗体的差异反应：CD45RO DNA 片段化和 CD45RO- 亚群增殖。
影响因子：
6.4
作者：
Li,J;Campbell,D;Hayward,AR
通讯作者：
Hayward,AR