Synthesis of fibronectin by the isolated perfused rat liver.
Synthesis of fibronectin by the isolated perfused rat liver.
复制标题
通过离体灌注大鼠肝脏合成纤连蛋白。
作者：
M. Owens;C. Cimino
血浆纤连蛋白合成的主要位点未知。使用离体灌注大鼠肝脏和抗大鼠纤连蛋白抗血清，我们已经测量了净肝合成纤连蛋白在10小时灌注期间。我们计算了体表面积为300平方厘米的195 g大鼠的循环血浆纤连蛋白池为3.3 mg：在10小时的肝灌注中观察到大鼠肝供体的净肝合成为3.75 +/-1.0mg/300平方厘米体表面积。当向肝灌注液中加入激素皮质醇和胰岛素时，观察到5.05 +/-0.82mg/300平方厘米的净合成。两种已知的急性期反应蛋白，纤维蛋白原和α-2急性期球蛋白的合成，也大大增加了皮质醇和胰岛素的存在。在皮下注射尿苔产生脓肿的完整大鼠中测量的血浆纤连蛋白水平为0.57 +/-0.05 mg/ml，而正常对照动物为0.38 +/- 0.02 mg/ml。我们的研究表明，肝合成实质上有助于血浆纤连蛋白池，并表明这种合成是由激素皮质醇和胰岛素增强。在完整的大鼠中，炎症与血浆纤连蛋白水平升高相关。
The major site(s) of synthesis of plasma fibronectin is unknown. Using the isolated perfused rat liver and anti-rat fibronectin antiserum, we have measured net hepatic synthesis of fibronectin during 10-hr perfusion periods. We calculated the circulating plasma fibronectin pool of a 195-g rat with body surface area of 300 sq cm to be 3.3 mg: net hepatic synthesis of 3.75 +/- 1.0 mg/300 sq cm body surface area of the rat liver donor was seen in liver perfusions of 10 hr. When the hormones cortisol and insulin were added to the liver perfusate, net synthesis of 5.05 +/- 0.82 mg/300 sq cm was seen. Synthesis of two known acute phase reactant proteins, fibrinogen and alpha-2 acute phase globulin, was also substantially greater in the presence of cortisol and insulin. Plasma fibronectin levels measured in intact rats that had abscess produced by subcutaneous turpentine injection were 0.57 +/- 0;05 mg/ml compared to 0.38 +/- 0.02 mg/ml in normal control animals. Our studies indicate that hepatic synthesis contributes substantially to the plasma fibronectin pool and show that such synthesis is enhanced by the hormones cortisol and insulin. In the intact rat, inflammation was associated with elevated levels of plasma fibronectin.