Second harmonic generation imaging microscopy studies of osteogenesis imperfecta
Second harmonic generation imaging microscopy studies of osteogenesis imperfecta
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DOI：
10.1117/1.2799538
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发表时间：
2007-09-01
影响因子：
3.5
通讯作者：
Campagnola, Paul J.
中科院分区：
文献类型：
--
作者：
Nadiarnykh, Oleg;Plotnikov, Sergey;Campagnola, Paul J.
我们使用定量二次谐波成像显微镜（SHG）研究了人类成骨不全症（OI）小鼠模型中胶原基质的组织。成骨不全是一种遗传性疾病，其中I型胶原原纤维组织异常或细小，导致临床表现为复发性骨折和其他与胶原组成组织相关的病理。利用SHG对超分子组装的敏感性，我们研究了这种方法是否可以用于区分正常组织和肿瘤组织。通过比较SHG强度、纤维形态、极化各向异性和信号方向性，我们发现在骨、肌腱和皮肤的野生型（WT）和疾病状态中获得了统计学上不同的结果。所有这些光学特征都与oim组织中胶原基质更加无序相一致，这些结果进一步与已知的oim小鼠较弱的力学性能相一致。虽然目前的工作显示SHG在小鼠模型中区分正常和病变状态的能力，但我们认为我们的结果为使用SHG作为人类OI的临床诊断工具提供了一个框架。我们进一步认为，所描述的SHG指标可以应用于其他以胶原蛋白异常聚集为特征的结缔组织疾病。(C) 2007光学仪器工程师学会。
We have used quantitative second harmonic generation (SHG) imaging microscopy to investigate the collagen matrix organization in the oim mouse model for human osteogenesis imperfecta (OI). OI is a heritable disease in which the type I collagen fibrils are either abnormally organized or small, resulting in a clinical presentation of recurrent bone fractures and other pathologies related to collagen-comprised tissues. Exploiting the exquisite sensitivity of SHG to supramolecular assembly, we investigated whether this approach can be utilized to differentiate normal and oim tissues. By comparing SHG intensity, fibrillar morphology, polarization anisotropy, and signal directionality, we show that statistically different results are obtained for the wild type (WT) and disease states in bone, tendon, and skin. All these optical signatures are consistent with the collagen matrix in the oim tissues being more disordered, and these results are further consistent with the known weaker mechanical properties of the oim mouse. While the current work shows the ability of SHG to differentiate normal and diseased states in a mouse model, we suggest that our results provide a framework for using SHG as a clinical diagnostic tool for human OI. We further suggest that the SHG metrics described could be applied to other connective tissue disorders that are characterized by abnormal collagen assembly. (C) 2007 Society of Photo-Optical Instrumentation Engineers.