Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model
Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model
复制标题
黄芪对卵白蛋白致过敏性鼻炎小鼠模型的影响
DOI：
10.1177/1945892419839259
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发表时间：
2019
影响因子：
2.6
通讯作者：
Liu Shi Xi
中科院分区：
文献类型：
--
作者：
Zhong Bing;Du Jin Tao;Liu Feng;Luo Ba;Liu Ya Feng;Liu Shi Xi
背景黄芪是一种传统的治疗变应性鼻炎的中药，但其治疗变应性鼻炎的机制尚不清楚。目的观察黄芪对变应性鼻炎的抗炎作用，探讨其抗变态反应的机制。方法用卵清蛋白诱导小鼠AR模型。分析过敏症状、鼻粘膜中嗜酸性粒细胞的数量和鼻灌洗液中炎性细胞的水平。我们检测了血清免疫球蛋白E（IgE）、白细胞介素-4（IL-4）、IL-5、IL-13、干扰素-γ（IFN-γ）和IL-10。此外，鼻黏膜组织中还检测到IL-4、IL-5和IL-13的相关mRNA。分析脾脏和鼻淋巴组织中CD 4 + CD 25 + Foxp 3 + T细胞的比例。检测了核因子-κ B p65（NF-κB p65）和抑制性κ B α（IκBα）的mRNA水平以及NF-κB p65 DNA结合活性。同时检测鼻黏膜NF-κB p65和p-NF-κB p65蛋白的表达。结果黄芪能减少鼻黏膜嗜酸性粒细胞的数量，降低鼻灌洗液中炎性细胞的含量。血清IgE、IL-4、IL-5和IL-13也降低，而IFN-γ和IL-10水平升高。AM可降低IL-4、IL-5和IL-13的相对mRNA水平。AM可增加脾脏和鼻淋巴组织中CD 4 + CD 25 + Foxp 3 + T细胞的比例。黄芪可通过抑制NF-κB p65的mRNA表达和DNA结合活性，降低NF-κ B的活性。AM对IκBα mRNA无明显影响。黄芪可降低鼻黏膜p-NF-κB p65蛋白的表达。结论黄芪可通过提高CD 4 + CD 25 + Foxp 3 + T细胞水平，抑制NF-κB B的活化，减少炎症细胞因子的分泌。
Background Astragalus membranaceus (AM), a traditional Chinese medicine, has been used to treat allergic diseases, but the mechanism for treating allergic rhinitis (AR) remains unclear. Objective The purpose of this study was to look at the anti-inflammatory effect of AM on AR and the mechanism of anti-allergy. Methods The mouse model of AR was induced by ovalbumin. Allergic symptoms, number of eosinophils in nasal mucosa, and levels of inflammatory cells in nasal lavage fluid were analyzed. We explored the serum immunoglobulin E (IgE), interleukin-4 (IL-4), IL-5, IL-13, interferon-γ (IFN-γ), and IL-10. Besides, the relative mRNA of IL-4, IL-5, and IL-13 was also detected in nasal mucosa tissue. The proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue were analyzed. The mRNA levels of nuclear factor-kappa B p65 (NF-κB p65) and inhibitory kappa B alpha (IκBα), as well as NF-κB p65 DNA binding activity, were tested. We also measured the protein levels of NF-κB p65 and p-NF-κB p65 in nasal mucosa. Results AM could reduce the number of eosinophils in the nasal mucosa and decrease the levels of inflammatory cells in nasal lavage fluid. The serum IgE, IL-4, IL-5, and IL-13 were also decreased, while levels of IFN-γ and IL-10 were increased. The relative mRNA of IL-4, IL-5, and IL-13 was decreased by AM. AM increased the proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue. In addition, AM could reduce the activity of NF-kB by inhibiting the mRNA expression and DNA binding activity of NF-κB p65. However, AM had no significant effect on mRNA of IκBα. Above all, AM could reduce the p-NF-κB p65 protein expression of nasal mucosa. Conclusions AM could reduce the secretion of inflammatory cytokines by increasing the level of CD4+ CD25+ Foxp3+ T cells and inhibiting the activation of the NF-κB.