The Coding Region of the HCV Genome Contains a Network of Regulatory RNA Structures.
The Coding Region of the HCV Genome Contains a Network of Regulatory RNA Structures.
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DOI：
10.1016/j.molcel.2016.01.024
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发表时间：
2016-04-07
期刊：
影响因子：
16
通讯作者：
Pyle AM
中科院分区：
文献类型：
--
作者：
Pirakitikulr N;Kohlway A;Lindenbach BD;Pyle AM
RNA 是一种多功能大分子，可容纳一级序列、二级和三级结构的功能信息。我们结合化学探测、RNA 结构建模、比较序列分析和功能测定来检查 RNA 结构在丙型肝炎病毒 (HCV) 基因组中的作用。我们描述了一组保守但功能多样的结构 RNA 基序，这些基序出现在 HCV 基因组的多个编码区中，并且我们证明这些基序的构象变化会影响病毒生命周期的特定阶段。我们的研究表明，这些类型的结构可以遍及基因组，发挥特定的机制和调节作用，构成控制生物过程的“代码中的代码”。
RNA is a versatile macromolecule that accommodates functional information in primary sequence, secondary and tertiary structure. We use a combination of chemical probing, RNA structure modeling, comparative sequence analysis and functional assays to examine the role of RNA structure in the hepatitis C virus (HCV) genome. We describe a set of conserved but functionally diverse structural RNA motifs that occur in multiple coding regions of the HCV genome, and we demonstrate that conformational changes in these motifs influence specific stages in the virus’s life cycle. Our study shows that these types of structures can pervade a genome, where they play specific mechanistic and regulatory roles, constituting a “code within the code” for controlling biological processes.