Adenovirus-2 messengers--an example of baroque molecular architecture.
Adenovirus-2 messengers--an example of baroque molecular architecture.
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腺病毒 2 信使——巴洛克分子结构的一个例子。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
B. Zain
中科院分区：
文献类型：
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作者：
T. Broker;L. Chow;A. Dunn;R. Gelinas;J. Hassell;D. Klessig;J. B. Lewis;R. Roberts;B. Zain
2型腺病毒（Ad2）可引起人类呼吸道感染，在人细胞系（如HeLa和KB）上高效生长，并可转化大鼠原代细胞系（Tooze 1973）。Ad2病毒体由一条长35，000碱基对（bp）（23 × 106道尔顿）的线性双链DNA染色体组成，包含在一个由至少10种不同蛋白质组成的二十面体衣壳中。穿透细胞膜后，病毒DNA和与之相关的几种核心蛋白被转运到细胞核，早期RNA转录在2小时内开始。感染后约8小时，病毒DNA开始复制，数小时后达到最大速率。后期转录产生衣壳和其他病毒特异性蛋白的信使RNA（mRNA）在DNA复制开始后开始，并持续约2天，此时感染的细胞死亡。
Adenovirus type 2 (Ad2) causes respiratory infections in humans, grows productively on human cell lines such as HeLa and KB, and can transform rat primary cell lines (Tooze 1973). The Ad2 virion consists of a linear duplex DNA chromosome 35,000 base pairs (bp) long (23 × 106 daltons) contained within an icosahedral capsid composed of at least ten different proteins. After penetration of the cell membrane, the viral DNA and several core proteins associated with it are transported to the nucleus, where early RNA transcription begins within 2 hours. About 8 hours after infection, viral DNA replication commences, reaching a maximum rate several hours later. Late transcription to produce messenger RNA (mRNA) for capsid and other virus-specific proteins begins after the onset of DNA replication and continues for about 2 days, when the infected cells die.