Automated electron tomography of the septal pore cap in Rhizoctonia solani
Automated electron tomography of the septal pore cap in Rhizoctonia solani
复制标题
DOI：
10.1006/jsbi.2000.4243
复制
发表时间：
2000-07-01
影响因子：
3
通讯作者：
Boekhout, T
中科院分区：
文献类型：
--
作者：
Müller, WH;Koster, AJ;Boekhout, T
丝状担子菌的中空孔隔和隔孔帽在维持菌丝细胞的完整性方面起着重要作用。本文研究了高压冷冻、冷冻置换和Spurr包埋处理后立枯丝核菌菌丝细胞中长孔隔膜和SPC的超微结构。我们可视化SPC与相关的细胞超微结构在三维厚切片细胞的自动电子断层扫描，然后进行三维断层重建。使用这些方法，我们能够记录线粒体通过SPC，小管状膜结构在隔孔通道的入口处，丝状结构连接的SPC的内侧与孔堵塞材料，细丝锚定的孔堵塞材料与质膜，小泡状体连接到堵塞材料，和管状内质网连续的SPC的基础。我们假设SPC、丝状结构、堵塞物质和内质网以协调的方式发挥作用，以维持丝状真菌R中的细胞完整性、细胞间通讯以及溶质和细胞器的运输。索拉尼(C)北京大学出版社.
Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hyphal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani. (C) 2000 Academic Press.