Interaction of certain cationic dyes with the respiratory chain of rat liver mitochondria.
Interaction of certain cationic dyes with the respiratory chain of rat liver mitochondria.
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DOI：
10.1016/s0021-9258(19)70150-1
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发表时间：
1981-01
期刊：
影响因子：
--
通讯作者：
T. E. Conover;R. F. Schneider
中科院分区：
文献类型：
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作者：
T. E. Conover;R. F. Schneider
已观察到花青型阳离子染料可特异性抑制大鼠肝线粒体中 NAD 相关的呼吸，在约 0.2 μmol/g 线粒体蛋白时可抑制 50%。在完全抑制 NADH 氧化的范围内，染料对琥珀酸氧化或偶联磷酸化没有影响。在所有测试的花青染料中都发现了这种特异性抑制作用，但是，除了派洛宁 B 之外，在其他阳离子染料（例如藏红 O、罗丹明染料）或喹啉鎓环结构的简单衍生物中没有观察到这种特异性抑制作用。观察到抑制作用具有时间和浓度依赖性，在 25°C 下，完全抑制的半衰期确定为 15 至 30 秒。此外，抑制作用完全依赖于底物氧化或 ATP 存在对线粒体膜的供能。花青染料抑制的能量依赖性解释为线粒体内与能量相关的染料浓度的简单要求，但与观察到的非常快的染料摄取速率相比，相对较慢的抑制起始并不支持这一解释。此外，观察到染料抑制了软骨下颗粒中需要能量的琥珀酸连接的 NAD 还原，而发现 NADH 引起的富马酸还原仅在除染料外还存在 ATP 的情况下才被显着抑制。线粒体下颗粒中电子传输抑制的能量需求表明，线粒体染料的能量依赖性积累不能单独解释花青染料抑制 NADH 氧化的能量需求。
Cationic dyes of the cyanine type have been observed to specifically inhibit NAD-linked respiration in rat liver mitochondria, with 50% inhibition occurring at about 0.2 mumol/g of mitochondrial protein. The dyes show no effect on succinate oxidation or coupled phosphorylation in the range which completely inhibits NADH oxidation. This specific inhibition was found with all cyanine dyes tested, but, with the possible exception of pyronin B, was not observed with other cationic dyes such as safranine O, rhodamine dyes, or with simple derivatives of the quinaldinium ring structure. The inhibition was observed to be both time- and concentration-dependent, with the half-time for full inhibition determined to be on the order of 15 to 30 s at 25 degrees C. Furthermore, the inhibition was totally dependent on the energization of the mitochondrial membrane by either substrate oxidation or the presence of ATP. The explanation of the energy dependence of the inhibition by cyanine dyes as the simple requirement for energy-linked dye concentration within the mitochondria is not supported by the relatively slow onset of inhibition as compared with the very rapid rate of dye uptake observed. Furthermore, inhibition of energy-requiring, succinate-linked NAD reduction in submitochondrial particles was observed to be inhibited by dyes, while fumarate reduction by NADH was found to be inhibited significantly only in the presence of ATP in addition to the dye. The energy requirement for electron transport inhibition in submitochondrial particles indicates that energy-dependent accumulation of the dyes by mitochondria cannot alone explain the energy requirement for the cyanine dye inhibition of NADH oxidation.