Composition, synthesis, and assembly of the embryonic chick retinal basal lamina
Composition, synthesis, and assembly of the embryonic chick retinal basal lamina
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DOI：
10.1006/dbio.2000.9649
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发表时间：
2000-04-15
影响因子：
2.7
通讯作者：
Cole, GJ
中科院分区：
文献类型：
--
作者：
Halfter, W;Dong, S;Cole, GJ
为了研究发育中的神经系统中的基底层的生物学，研究了胚胎视网膜基底层的蛋白质组成，确定了其蛋白质在眼中的合成位点，并在两种测定系统中在体内研究了基底层组装。层粘连蛋白、巢蛋白、聚集蛋白、胶原IV和XVIII是视网膜基底层的主要成分。然而，只有聚集蛋白由视网膜合成，而其他基质成分来源于睫状体、透镜或视盘的细胞。从视网膜外组织的合成推断，视网膜基底层蛋白必须从其来源组织脱落到玻璃体中，并从那里结合到视网膜神经上皮提供的受体蛋白。视网膜基底层的所有典型蛋白质在玻璃体中是丰富的，并且只有当玻璃体直接邻近视网膜时才形成新的基底层，这一事实与玻璃体在视网膜基底层形成中具有功能的论点一致。还研究了通过眼内注射胶原酶破坏视网膜基底膜后基底膜的组装。胶原酶抑制剂Matrigel作用于胶原酶后，基底膜再生。基底层在6 h内重建。然而，再生的基底层位于更深的视网膜比正常的重建沿着缩回的神经上皮终足，表明这些终足是基底层组件的首选网站。(C)北京大学出版社.
To study the biology of basal laminae in the developing nervous system the protein composition of the embryonic retinal basal lamina was investigated, the site of synthesis of its proteins in the eye was determined, and basal lamina assembly was studied in vivo in two assay systems. Laminin, nidogen, agrin, collagen IV, and XVIII are major constituents of the retinal basal lamina. However, only agrin is synthesized by the retina, whereas the other matrix constituents originate from cells of the ciliary body, the lens, or the optic disc. The synthesis from extraretinal tissues infers that the retinal basal lamina proteins must be shed from their tissues of origin into the vitreous body and from there bind to receptor proteins provided by the retinal neuroepithelium. The fact that all proteins typical for the retinal basal lamina are abundant in the vitreous body and a new basal lamina is only formed when the vitreous body was directly adjacent to the retina is consistent with the contention of the vitreous body having a function in retinal basal lamina formation. Basal lamina assembly was also studied after disrupting the retinal basal lamina by intraocular injection of collagenase. The basal lamina regenerated after chasing the collagenase with Matrigel, which served as a collagenase inhibitor. The basal lamina was reconstituted within 6 h. However, the regenerated basal lamina was located deeper in the retina than normal by reconstituting along the retracted neuroepithelial endfeet demonstrating that these endfeet are the preferred site of basal lamina assembly. (C) 2000 Academic Press.