DELTA-AMINOLEVULINIC-ACID DEHYDRATASE ASSAY
DELTA-AMINOLEVULINIC-ACID DEHYDRATASE ASSAY
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DOI：
10.1159/000459097
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
SASSA, S
中科院分区：
文献类型：
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作者：
SASSA, S
δ-氨基乙酰丙酸（ALA）水解酶催化2分子ALA合成胆色素原（PBG）。本文提出了一种半微量比色法测定各种组织中的丙氨酸转氨酶。对于用或不用二硫苏糖醇测定的匀浆，成年雄性大鼠肝脏中的酶活性分别为2.22和1.94 μ mol形成的PBG/g肝脏/h。至少2.5 h和高达2.5 mg组织/试验的试验呈线性。ALA的Km为4.0 ×10-4 M，最适pH为6.2 - 6.4。讨论了激活剂和抑制剂对酶活性的影响。
.delta.-Aminolevulinic acid (ALA) dehydratase catalyzes the synthesis of porphobilinogen (PBG) from 2 molecules of ALA. A semimicro method for the colorimetric determination of ALA dehydratase is presented and applied to various tissues. The enzyme activity in adult male rat liver was 2.22 and 1.94 .mu.mol PBG formed/g liver per h for homogenates assayed with or without dithiothreitol, respectively. The assay was linear for at least 2.5 h and for up to 2.5 mg tissue/assay. The Km for ALA was 4.0 .times. 10-4 M and the pH optimum was 6.2-6.4. The effects of activators and inhibitors on enzyme activity are discussed.