Hepatitis C virus NS5A protein is phosphorylated by casein kinase II
Hepatitis C virus NS5A protein is phosphorylated by casein kinase II
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DOI：
10.1006/bbrc.1999.0460
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发表时间：
1999-04-21
影响因子：
3.1
通讯作者：
Choe, J
中科院分区：
文献类型：
--
作者：
Kim, J;Lee, D;Choe, J
丙型肝炎病毒（HCV）具有编码多聚蛋白的正链RNA基因组，所述多聚蛋白被细胞和病毒蛋白酶后加工成三种结构蛋白和六种非结构（NS）蛋白。在哺乳动物细胞中表达的NS 5A蛋白以56 kDa和58 kDa的两种磷酸化形式存在。在这项研究中，我们提供的证据NS 5A和蛋白激酶从大鼠-1细胞的亲和固定谷胱甘肽-S-转移酶（GST）-NS 5A融合蛋白之间的稳定协会。该蛋白激酶通过NS 5A的N-末端结合，不受细胞周期调节。GST-NS 5A也在体外被纯化的酪蛋白激酶II（CKII）（CMCG激酶家族的成员）磷酸化。由于CKII和NS 5A相关蛋白激酶具有相同的分子大小和性质，通过凝胶内激酶试验和抑制剂处理试验，我们得出结论，HCV NS 5A蛋白被CKII磷酸化。(C)北京：科学出版社.
Hepatitis C virus (HCV) has a positive-strand RNA genome that encodes a polyprotein, which is posttranslationally processed by cellular and viral proteinases into three structural and six non-structural (NS) proteins. The NS5A protein, expressed in mammalian cells, exists as two phosphorylated forms of 56 kDa and 58 kDa. In this study, we provide evidence for a stable association between NS5A and a protein kinase from rat-1 cells by affinity to immobilized glutathione-S-transferase (GST)-NS5A fusion protein. This protein kinase was associated through the N-terminus of NS5A and was not regulated by cell cycle. The GST-NS5A was also phosphorylated in vitro by the purified casein kinase II (CKII), a member of the CMCG kinase family. Since CKII and the NS5A-associated protein kinase have the same molecular size and property by In-gel kinase assay and an inhibitor treatment test, we conclude that HCV NS5A protein is phosphorylated by CKII. (C) 1999 Academic Press.