Triggered release of proteins from emulsan-alginate beads
Triggered release of proteins from emulsan-alginate beads
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DOI：
10.1016/j.jconrel.2005.09.042
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发表时间：
2005-12-05
影响因子：
10.8
通讯作者：
Kaplan, DL
中科院分区：
文献类型：
--
作者：
Castro, GR;Kamdar, RR;Kaplan, DL
Emulsan/藻酸盐珠粒的蛋白质吸附和稳定性的背景下，控制释放进行了研究。该珠粒直径为400 +/-80 μ m，含有约10%的emulsan，由于emulsan结合蛋白质的天然能力，加上这种复杂脂杂多糖的选择性生物活化特征，为蛋白质的递送提供了不寻常的机会。emulsan/藻酸盐珠对偶氮-牛血清白蛋白的结合能力为0.637 +/-0.004 μ g/mg，而单独由藻酸盐形成的珠为0.170 +/-0.007 μ g/mg。在另外的蛋白质吸附实验中，脂肪酶和枯草杆菌蛋白酶在吸附到emulsan/藻酸盐珠粒时保持活性，尽管与溶液中游离的酶相比具有较低的比活性。然而，吸附酶的半衰期显着高于游离形式。为了探索该系统的功能效用，在这些珠系统的背景下研究了两种类型的触发释放。首先，偶氮-BSA作为模型蛋白物理结合到emulsan/藻酸盐珠，然后通过用枯草杆菌蛋白酶（一种丝氨酸蛋白酶，其从结合的蛋白质切割偶氮染料磺胺酸）触发而选择性地释放。在不存在枯草杆菌蛋白酶的情况下，未观察到触发释放。其次，偶氮-BSA作为前药模型，吸附到emulsan/藻酸盐珠，然后通过脂肪酶处理证明染料的释放，所述脂肪酶处理将脂肪酸酯从emulsan结构中裂解以释放结合的蛋白质。结果确立了基于乳胶蛋白的珠粒用于蛋白质结合和触发释放的多功能性和实用性。(c)2005 Elsevier B. V.保留所有权利。
Emulsan/alginate beads were studied for protein adsorption and stability in the context of controlled release. The beads, 400 +/- 80 mu m diameter with approximately 10% emulsan content, offer unusual opportunities for delivery of proteins due to the natural ability of emulsan to bind proteins, coupled with the selective biological activation features of this complex lipoheteropolysaccharide. The binding capacity of azo-bovine serum albumin by the emulsan/alginate beads was 0.637 +/- 0.004 vs. 0.170 +/- 0.007 mu g/mg for beads formed from alginate alone. In additional protein adsorption experiments, the lipase and subtilisin maintained activity when adsorbed to the emulsan/alginate beads albeit with lower specific activity when compared to the enzyme free in solution. However, the half life of the adsorbed enzyme was significantly higher than the free forms. To explore functional utility of this system, two types of triggered release were studied in the context of these bead systems. First, azo-BSA as a model protein was physically bound to emulsan/alginate beads and then selectively released by triggering with subtilisin, a serine protease, which cleaves the azo dye, sulfanilic acid, from the bound protein. In absence of subtilisin no triggered release was observed. Second, azo-BSA as a prodrug model, was adsorbed to the emulsan/alginate beads and then release of the dye was demonstrated by lipase treatment which cleaves the fatty acid esters from the emulsan structure to release the bound protein. The results establish the versatility and utility of emulsan-based beads for protein binding and triggered release. (c) 2005 Elsevier B.V. All rights reserved.