Detecting Sonolysis of Polyethylene Glycol Upon Functionalizing Carbon Nanotubes.
Detecting Sonolysis of Polyethylene Glycol Upon Functionalizing Carbon Nanotubes.
复制标题
检测功能化碳纳米管后聚乙二醇的声分解。
DOI：
10.1007/978-1-4939-6646-2_10
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Draper,Rockford
中科院分区：
文献类型：
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作者：
Wang,Ruhung;Murali,VasanthS;Draper,Rockford
聚乙二醇（PEG）和相关聚合物通常用于通过超声处理碳纳米材料的增溶和非共价功能化。例如，经常用 Pluronic® 系列的含 PEG 表面活性剂或磷脂-PEG 聚合物对碳纳米管进行超声处理，以非共价功能化纳米管。然而，PEG 对超声降解非常敏感，并且降解产物可能对哺乳动物细胞和斑马鱼胚胎等生物体有毒。因此，如本章所述，有一种简单且廉价的方法来确定潜在 PEG 超声溶解的程度是有用的。通过电泳在十二烷基硫酸钠聚丙烯酰胺凝胶上解析完整的 PEG 聚合物和降解的片段，并通过碘化钡 (BaI2) 染色进行可视化。使用平板照片扫描仪获取凝胶的数字化图像，并使用 ImageJ 软件量化 BaI2 染色的 PEG 带的强度。与未经超声处理的完整 PEG 聚合物相比，通过凝胶中条带强度的降低，可以轻松检测到超声处理后 PEG 聚合物的降解。此外，该方法还可用于快速筛选各种超声处理条件，以确定那些可以将 PEG 降解降至可接受水平的条件。
Polyethylene glycol (PEG) and related polymers are often used in the solubilization and noncovalent functionalization of carbon nanomaterials by sonication. For example, carbon nanotubes are frequently sonicated with PEG-containing surfactants of the Pluronic®series or phospholipid-PEG polymers to noncovalently functionalize the nanotubes. However, PEG is very sensitive to degradation upon sonication and the degradation products can be toxic to mammalian cells and to organisms such as zebrafish embryos. It is therefore useful to have a simple and inexpensive method to determine the extent of potential PEG sonolysis, as described in this chapter. Intact PEG polymers and degraded fragments are resolved on sodium dodecyl sulfate polyacrylamide gels by electrophoresis and visualized by staining with barium iodine (BaI2). Digitized images of gels are acquired using a flatbed photo scanner and the intensities of BaI2-stained PEG bands are quantified usingImageJsoftware. Degradation of PEG polymers after sonication is readily detected by the reduction of band intensities in gels compared to those of non-sonicated, intact PEG polymers. In addition, the approach can be used to rapidly screen various sonication conditions to identify those that might minimize PEG degradation to acceptable levels.