A Member of an Ancient Family of Bacterial Amino Acids Transporters Contributes to Chlamydia Nutritional Virulence and Immune Evasion.
A Member of an Ancient Family of Bacterial Amino Acids Transporters Contributes to Chlamydia Nutritional Virulence and Immune Evasion.
复制标题
细菌氨基酸转运蛋白古老家族的成员有助于衣原体的营养毒力和免疫逃避。
DOI：
10.1128/iai.00483-22
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发表时间：
2023
影响因子：
3.1
通讯作者：
Nelson,DavidE
中科院分区：
文献类型：
--
作者：
Banerjee,Arkaprabha;Sun,Yuan;Muramatsu,MatthewK;Toh,Evelyn;Nelson,DavidE
许多专性细胞内细菌，包括衣原体属的成员，不能合成各种氨基酸，并通过很大程度上未知的机制从宿主细胞获得这些氨基酸。先前，我们确定了一个错义突变inctl0225，一个功能未知的保守衣原体开放阅读框，介导了对干扰素γ的敏感性。本研究表明，CTL0225是中性氨基酸转运体SnatA家族的一员，该转运体有助于将几种氨基酸导入衣原体细胞。此外，我们发现CTL0225同源物来自另外两种远亲专性细胞内病原体（伯纳氏杆菌和蚜虫布氏杆菌），足以将缬氨酸导入大肠杆菌。我们还发现衣原体感染和干扰素暴露对氨基酸代谢有相反的影响，这可能解释了CTL0225与干扰素敏感性之间的关系。总的来说，我们表明，系统发育多样化的细胞内病原体使用一个古老的氨基酸转运蛋白家族来获取宿主氨基酸，并提供了另一个例子，说明营养毒力和免疫逃避如何在专性细胞内病原体中联系起来。
Many obligate intracellular bacteria, including members of the genus Chlamydia, cannot synthesize a variety of amino acidsde novoand acquire these from host cells via largely unknown mechanisms. Previously, we determined that a missense mutation inctl0225, a conserved Chlamydia open reading frame of unknown function, mediated sensitivity to interferon gamma. Here, we show evidence that CTL0225 is a member of the SnatA family of neutral amino acid transporters that contributes to the import of several amino acids into Chlamydia cells. Further, we show that CTL0225 orthologs from two other distantly related obligate intracellular pathogens (Coxiella burnetii and Buchnera aphidicola) are sufficient to import valine into Escherichia coli. We also show that chlamydia infection and interferon exposure have opposing effects on amino acid metabolism, potentially explaining the relationship between CTL0225 and interferon sensitivity. Overall, we show that phylogenetically diverse intracellular pathogens use an ancient family of amino acid transporters to acquire host amino acids and provide another example of how nutritional virulence and immune evasion can be linked in obligate intracellular pathogens.