Periosteal remodeling at the femoral neck in nonhuman primates
Periosteal remodeling at the femoral neck in nonhuman primates
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DOI：
10.1359/jbmr.060414
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发表时间：
2006-07-01
影响因子：
6.2
通讯作者：
Orwoll, E
中科院分区：
文献类型：
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作者：
Bliziotes, M;Sibonga, JD;Orwoll, E
骨尺寸是骨强度的重要决定因素，骨膜表面的细胞事件可以改变骨尺寸。我们通过对非人灵长类动物股骨颈和股骨轴的动态组织形态学研究来表征骨膜细胞活动。材料与方法：对16只完整成年雌雄非人灵长类动物（恒河猴[Macaca mulatta， n = 9]和日本猕猴[Macaca fuscata， n = 7]）的股骨标本进行分析。动物用四环素双标记，最后一次给药后2-7天进行尸检。我们在另外一组5只完整的和4只阉割的雌性动物中描述了骨膜吸收活动。在完整动物中进行多组比较，采用单因素方差分析，然后进行Fisher PLSD后检验。在去性腺的动物中，对二分类采用Fisher精确检验，对连续变量采用Mann-Whitney u检验。结果：股骨颈骨膜的骨更新比股骨干快，但比股骨颈松质骨慢。同样，在这些完整的动物中，股骨颈骨膜表面皮质骨的侵蚀面明显大于松质骨室（p < 0.0001）或股骨干（p < 0.0001）。性腺去角质雌性动物骨膜表面破骨细胞数量明显高于正常对照组（p < 0.01）。结论：我们通过组织形态学分析记录了股骨颈的膜内骨膜骨转换。随着时间的推移，组织水平的骨形成率足以实质性地增加股骨颈的大小。骨膜破骨活性不是骨表面出现皮质内隧道的结果。性类固醇缺乏导致骨膜表面破骨细胞数量增加。这是股骨颈骨膜翻转的第一个系统文献。
Introduction: Bone size is an important determinant of bone strength, and cellular events at the periosteal surface could alter bone dimensions. We characterized periosteal cellular activity with dynamic histomorphometric studies of nonhuman primate femoral neck and shaft.Materials and Methods: Femur specimens from 16 intact adult male and female nonhuman primates (Rhesus [Macaca mulatta, n = 9] and Japanese Macaque [Macaca fuscata, n = 7]) were analyzed. Animals were double-labeled with tetracycline, and necropsy was performed 2-7 days after the last dose. We characterized periosteal resorptive activity in an additional group of five intact and four castrate female animals. Multiple group comparisons in intact animals were performed by one-way ANOVA followed by a Fisher PLSD posthoc test. In gonadectomized animals, Fisher's exact test was used for dichotomous and Mann-Whitney U-test for continuous variables.Results: Bone turnover in the periosteum of the femoral neck in intact animals was more rapid than at the femoral shaft but slower than in femoral neck cancellous bone. Similarly, in these intact animals, the eroded surface of cortical bone at the femoral neck periosteal surface was significantly greater than in the cancellous bone compartment (p < 0.0001) or on the femoral shaft (p < 0.0001). Gonadectomized female animals showed an increase in osteoclast number on the periosteal surface compared with intact controls (p < 0.01).Conclusions: We documented intramembranous periosteal bone turnover in the femoral neck by histomorphometric analyses. The tissue level bone formation rate was sufficient to add substantively to femoral neck size over time. Periosteal osteoclastic activity was not the result of the emergence of intracortical tunneling at the bone surface. Sex steroid deficiency produced an increase in osteoclast numbers at the periosteal surface. This is the first systematic documentation of periosteal turnover at the femoral neck.