RESPONSES OF CULTURED TOBACCO CELLS TO CRYPTOGEIN, A PROTEINACEOUS ELICITOR FROM PHYTOPHTHORA-CRYPTOGEA - POSSIBLE PLASMALEMMA INVOLVEMENT
RESPONSES OF CULTURED TOBACCO CELLS TO CRYPTOGEIN, A PROTEINACEOUS ELICITOR FROM PHYTOPHTHORA-CRYPTOGEA - POSSIBLE PLASMALEMMA INVOLVEMENT
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DOI：
10.1104/pp.95.2.486
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发表时间：
1991-02-01
期刊：
影响因子：
7.4
通讯作者：
RICCI, P
中科院分区：
文献类型：
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作者：
BLEIN, JP;MILAT, ML;RICCI, P
在培养中，植物病原真菌隐菌疫霉 (Phytophthora cryptogea) 分泌一种蛋白质，可引起过敏样坏死，并保护烟草植物免受病原体寄生疫霉 (Phytophthora parasitica var. var.) 的入侵。烟草亚科。 这种蛋白质被命名为隐黄蛋白，已被纯化并确定了其氨基酸序列。 在这项工作中，我们研究了隐黄素对烟草细胞悬浮培养物的影响。 隐黄素在大约 0.10 微摩尔浓度时是致命的。 当以亚致死剂量添加时，它会引起乙烯和植物抗毒素的产生。 它还诱导细胞外介质的 pH 值和电导率快速增加，而不影响质膜的完整性。 隐黄菌素减少了梭菌素诱导的细胞外培养基的酸化。 抑制梭菌素反应50%的浓度为0.8纳摩尔，而需要1微摩尔的赤藓红B（一种ATP酶抑制剂）才能产生相同的抑制作用。 然而，隐黄蛋白并不抑制纯化质膜 ATP 酶的活性。 全细胞结合研究的结果表明存在对隐黄素具有高亲和力的激发子结合位点。 讨论了质膜在激发子和细胞之间最初相互作用过程中的参与。
In culture, the phytopathogenic fungus Phytophthora cryptogea secretes a protein which elicits hypersensitive-like necroses and protects tobacco plants against invasion by the pathogen Phytophthora parasitica var. nicotianae. This protein, named cryptogein, has been purified and its amino acid sequence determined. In this work, we studied the effect of cryptogein on tobacco cell suspension cultures. Cryptogein was lethal at about 0.10 micromolar. When added at sublethal doses, it elicited the production of ethylene and phytoalexins. It also induced a rapid increase in pH and conductivity of the extracellular medium without affecting the integrity of the plasma membrane. Cryptogein reduced the fusicoccin-induced acidification of the extracellular medium. The concentration which inhibited the fusicoccin response by 50% was 0.8 nanomolar, while 1 micromolar erythrosine B, an ATPase inhibitor, was needed to produce the same inhibition. However, cryptogein did not inhibit the activity of a purified plasma membrane ATPase. Results of binding studies with whole cells suggested the presence of elicitor-binding sites with a high affinity for cryptogein. The involvement of the plasma membrane during the initial interaction between elicitor and cells is discussed.