A device for dried blood microsampling in quantitative bioanalysis: overcoming the issues associated with blood hematocrit
A device for dried blood microsampling in quantitative bioanalysis: overcoming the issues associated with blood hematocrit
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DOI：
10.4155/bio.14.310
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发表时间：
2015-01-01
期刊：
影响因子：
1.8
通讯作者：
Rudge, James B.
中科院分区：
文献类型：
--
作者：
Spooner, Neil;Denniff, Philip;Rudge, James B.
目的：对一种新型的干血采样器进行跨实验室实验，以研究它是否克服了从干血斑点取样时观察到的与血容量和红细胞压积(HCT)相关的问题。材料与方法：受试者平均吸收10.6%的L血，其吸收率为20~65%。结果：在Hct范围内，吸收体积没有明显变化。此外，六个不同实验室的血样数量的差异在可接受的范围内。结论：新型体积吸收微量采样器具有干血斑采样的优点，同时克服了与该技术相关的一些问题。
Aims: A cross-laboratory experiment has been performed on a novel dried blood sampler in order to investigate whether it overcomes issues associated with blood volume and hematocrit (HCT) that are observed when taking a subpunch from dried blood spot samples. Materials & methods: An average blood volume of 10.6 l was absorbed by the samplers across the different HCTs investigated (20-65%). Results: No notable change of volume absorbed was noted across the HCT range. Furthermore, the variation in blood sample volumes across six different laboratories was within acceptable limits. Conclusion: The novel volumetric absorptive microsampling device has the potential to deliver the advantages of dried blood spot sampling while overcoming some of the issues associated with the technology.