The complete mitogenomes of six higher termite species reconstructed from metagenomic datasets (Cornitermes sp., Cubitermes ugandensis, Microcerotermes parvus, Nasutitermes corniger, Neocapritermes taracua, and Termes hospes)
The complete mitogenomes of six higher termite species reconstructed from metagenomic datasets (Cornitermes sp., Cubitermes ugandensis, Microcerotermes parvus, Nasutitermes corniger, Neocapritermes taracua, and Termes hospes)
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DOI：
10.3109/19401736.2014.987257
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Brune, Andreas
中科院分区：
文献类型：
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作者：
Dietrich, Carsten;Brune, Andreas
我们重建了完整的线粒体基因组的六个高等白蚁物种从宏基因组数据集的隔离后肠车厢。检索测序读段，并使用诱饵和迭代映射算法（MITObim）进行组装，该算法产生封闭的有丝分裂基因组，无需额外的整理工作（平均覆盖范围为2300- 17，000倍）。基因组大小为16.1至17.6 kbp，G+C含量为32至35摩尔%;每个基因组包含相同的37个基因，也存在于其他白蚁物种的线粒体中。我们的研究大大增加了可用于系统发育研究的白蚁有丝分裂基因组的数量，并提供了一种简便的策略，用于在相关微生物群的宏基因组研究中识别宿主物种。
We reconstructed the complete mitochondrial genomes of six higher termite species from metagenomic datasets of their isolated hindgut compartments. The sequencing reads were retrieved and assembled with the mitochondrial-baiting and iterative-mapping algorithm (MITObim), which yielded closed mitogenomes without additional finishing efforts (average coverage ranging from 2300- to 17,000-fold). The genomes ranged from 16.1 to 17.6 kbp in size and had G+C contents between 32 and 35 mol%; each contained the same 37 genes present also in the mitochondria of other termite species. Our study substantially increases the number of termite mitogenomes available for phylogenetic studies and offers a facile strategy for identifying host species in metagenomic studies of their associated microbiota.