Expression of N-cadherin and alpha-catenin in astrocytomas and glioblastomas.
Expression of N-cadherin and alpha-catenin in astrocytomas and glioblastomas.
复制标题
N-钙粘着蛋白和α-钙蛋白在星形胶质细胞瘤和胶质母细胞瘤中的表达。
DOI：
10.1038/bjc.1995.384
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发表时间：
1995-09
影响因子：
8.8
通讯作者：
Hirohashi, S
中科院分区：
文献类型：
--
作者：
Shinoura, N;Paradies, N E;Warnick, R E;Chen, H;Larson, J J;Tew, J J;Simon, M;Lynch, R A;Kanai, Y;Hirohashi, S
我们检测了一系列胶质瘤和胶质母细胞瘤细胞系中N-钙粘蛋白（神经组织中主要的钙粘蛋白）的mRNA和蛋白水平，以及钙粘蛋白相关蛋白α-连环蛋白的mRNA水平。成胶质细胞瘤中N-钙粘蛋白和α-连环蛋白的mRNA水平显著高于低级别星形细胞瘤或正常脑，而成胶质细胞瘤、低级别星形细胞瘤和脑中完整的N-钙粘蛋白水平相似。此外，在相同组织病理学级别的高度侵袭性肿瘤与微创肿瘤中，侵袭性与N-钙粘蛋白和α-连环蛋白的表达之间没有一致的关系。为了进一步评估钙粘蛋白表达和神经肿瘤侵袭之间的关系，我们测量了N-钙粘蛋白的表达，钙依赖性细胞粘附和几个胶质母细胞瘤细胞系的运动。虽然所有的N-钙粘蛋白表达线的粘合剂，N-钙粘蛋白的表达水平和细胞运动之间没有相关性。总之，这些发现意味着，在由E-钙粘蛋白在癌中发挥的作用相反，N-钙粘蛋白不限制胶质母细胞瘤的侵袭。
We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.