X-RAY-MICROANALYSIS OF PLASMODIUM-FALCIPARUM AND INFECTED RED BLOOD-CELLS - EFFECTS OF QINGHAOSU AND CHLOROQUINE ON POTASSIUM, SODIUM, AND PHOSPHORUS COMPOSITION
X-RAY-MICROANALYSIS OF PLASMODIUM-FALCIPARUM AND INFECTED RED BLOOD-CELLS - EFFECTS OF QINGHAOSU AND CHLOROQUINE ON POTASSIUM, SODIUM, AND PHOSPHORUS COMPOSITION
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DOI：
10.4269/ajtmh.1988.39.157
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发表时间：
1988-08-01
影响因子：
3.3
通讯作者：
KIRK, RG
中科院分区：
文献类型：
--
作者：
LEE, P;YE, ZG;KIRK, RG
用能量色散X射线微区分析法对感染恶性疟原虫的人红细胞冰冻切片进行分析，以分别确定疟原虫及其红细胞宿主的元素组成。研究了两种抗疟药，青蒿素和氯喹，对钾，钠，磷浓度的影响。疟疾感染导致宿主红细胞中钾浓度降低和钠浓度升高。然而，阳离子组成的剧烈变化仅发生在被晚期寄生虫（晚期滋养体和寄生体）感染的红细胞中。被早期寄生虫（环期）感染的红细胞仅显示钠浓度的微小变化。此外，寄生培养物中未感染的红细胞与正常红细胞的组成没有差异。用青蒿素（10 ~（-6）M）或氯喹（10 ~（-6）M）处理8小时，可使早、晚寄生虫体内磷浓度下降。用青蒿素处理8小时还可使早期和晚期寄生虫的钾浓度降低并使钠浓度增加。相反，用氯喹处理8小时仅引起晚期寄生虫的钠和钾浓度的变化，而不影响早期寄生虫。
Cryosections of human red blood cells infected by Plasmodium falciparum were analyzed by energy dispersive x-ray microanalysis to determine the elemental composition of the parasites and their red cell hosts separately. The effects of two antimalarial drugs, quinghaosu and chlorquine, on potassium, sodium, and phosphorus concentrations were studied. Malarial infection causes a decrease in potassium concentration and an increase in sodium concentration in the host red cells. The drastic change in the cation composition, however, occurs only in red cells infected by late stage parasites (late trophozoite and schizont). Red cells infected by early stage parasites (ring stage) show only small changes in sodium concentration. Furthermore, the noninfected red cells in parasitized cultures show no difference in composition from those of normal red cells. Treatment of the parasitized cultures with qinghaosu (10-6M) or chloroquine (10-6M) for 8 hr causes phosphorus concentration of both early and late parasites to decrease. An 8 hr treatment with qinghaosu also produces a reduction in potassium and an increase in sodium concentrations in early and late parasites. In contrast, 8 hr treatment with chloroquine only causes a change in the sodium and potassium concentrations of the late stage parasites and does not affect the early stage parasites.