Genetic diversity in the endangered Camellia nitidissima assessed using transcriptome-based SSR markers
Genetic diversity in the endangered Camellia nitidissima assessed using transcriptome-based SSR markers
复制标题
使用基于转录组的 SSR 标记评估濒危金花茶的遗传多样性
DOI：
10.1007/s00468-019-01935-1
复制
发表时间：
2020-04-01
影响因子：
2.3
通讯作者：
Yin, Heng-fu
中科院分区：
文献类型：
--
作者：
Li, Xin-lei;Wang, Jie;Yin, Heng-fu
山茶是一种濒临灭绝的物种，它的花是亮黄色的。近50年来，气候和人类活动引起的生境变化使黑桫椤自然居群的范围和规模大大减少。本文利用SSR （simple sequence repeats）标记研究了黑桫椤自然居群的遗传多样性和遗传结构，为该植物的保护和利用提供基础依据。通过对不同山茶品种的多个转录组进行比较分析，获得了一组一致的转录序列，用于标记开发。我们筛选了237个预测转录位点，并鉴定出21个高多态性SSR标记用于群体分析。共获得82个等位基因，用于评价广西壮族自治区4个自然种群的遗传多样性。结果表明，各群体的平均观察杂合度（Ho）和期望杂合度（He）分别为0.624和0.546，遗传多样性主要存在于群体间。此外，我们还发现了蚁群遗传距离与地理距离之间存在显著的正相关关系。
Camellia nitidissima is an endangered species whose flowers are bright yellow. In the past 50 years, habitat changes caused by climate and human activity have greatly reduced the extent and size of natural populations of C. nitidissima. Here, to provide a fundamental basis for this plant’s protection and utilization, the genetic diversity and genetic structure of C. nitidissima natural populations were studied using simple sequence repeats (SSR) markers. Based on a comparative analysis of multiple transcriptomes from different Camellia species, a group of consensus transcript sequences were obtained for marker development. We screened 237 predicted transcript loci, and identified 21 highly polymorphic SSR markers for population analysis. In total, 82 alleles were obtained to assess the genetic diversity of four natural populations in Guangxi Zhuang autonomous region, China. The results revealed that the mean observed heterozygosity (Ho) and expected heterozygosity (He) of the populations were 0.624 and 0.546, respectively, and the genetic diversity was mostly among populations. Further, we uncovered a significant positive correlation between the genetic distance and geographic distance of C. nitidissima populations.