Functional characterization of the translationally controlled tumor protein (TCTP) gene associated with growth and defense response in cabbage
Functional characterization of the translationally controlled tumor protein (TCTP) gene associated with growth and defense response in cabbage
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DOI：
10.1007/s11240-010-9769-6
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发表时间：
2010-11-01
影响因子：
3
通讯作者：
Lei, Jianjun
中科院分区：
文献类型：
--
作者：
Cao, Bihao;Lu, Yaqing;Lei, Jianjun
翻译控制的肿瘤蛋白(TCTP)是雷帕霉素(TOR)信号通路靶标的重要组成部分，TOR是动物和真菌细胞生长的主要调节因子。尽管与之相关，但关于植物TCTP同源物的知识仍然有限。本研究从甘蓝(Brassica oleracea L.)C DNA文库。BoTCTP基因编码168个氨基酸的多肽，在所分析的所有物种中共享高度保守的GTP酶结合表面。Northern印迹分析表明，BoTCTP在根和茎中特异表达。此外，包括高温和盐胁迫在内的胁迫刺激都能明显增强BoTCTP的表达，而ABA胁迫下BoTCTP的表达没有明显变化。通过RNA干扰(RNAi)沉默BoTCTP进行了功能分析，发现BoTCTP RNAi植株的营养生长速率降低，对低温、高温和盐胁迫的耐受性降低。已报道的结果清楚地表明BoTCTP基因参与了甘蓝生长和逆境反应的调节。
The translationally controlled tumor protein (TCTP) is an important component of the target of rapamycin (TOR) signaling pathway, the major regulator of cell growth in animals and fungi. Despite its relevance, knowledge on plant TCTP homologs is still limited. In the present study, the full-length BoTCTP cDNA was isolated from a cabbage (Brassica oleracea L.) cDNA library. The BoTCTP cDNA encodes a polypeptide of 168 amino acids and shared the highly conserved GTPase binding surface in all of the species analyzed. Northern blotting analysis showed that BoTCTP was specifically expressed in the root and stem. Furthermore, the expression of BoTCTP could be obviously enhanced by stress stimuli, including high temperature and salt stresses, while no significant changes in the BoTCTP expression were observed under ABA stress. Functional analysis of BoTCTP was performed by the silencing of BoTCTP using RNA interference (RNAi) and the BoTCTP RNAi plants exhibited reduced vegetative growth rate and decreased tolerance of the cold, high temperature, and salt stresses. The reported results clearly suggest that the BoTCTP gene is involved in the regulation of both growth and stress response in cabbage.