Marine Actinobacteria from the Gulf of California: diversity, abundance and secondary metabolite biosynthetic potential.
Marine Actinobacteria from the Gulf of California: diversity, abundance and secondary metabolite biosynthetic potential.
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DOI：
10.1007/s10482-012-9863-3
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Soria-Mercado IE
中科院分区：
文献类型：
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作者：
Becerril-Espinosa A;Freel KC;Jensen PR;Soria-Mercado IE
加州湾是一个生物资源丰富、特有性高的沿海海洋生态系统。在这项工作中，我们报告了放线菌的分离和表征，从不同的网站在西部的加州湾。我们收集了126个沉积物样品，从每个样品中平均分离出3.1-38.3株放线菌。对136株菌株进行系统发育分析，鉴定为马杜拉放线菌属、小单孢菌属、拟诺卡氏菌属、野村菌属、单孢菌属、盐孢菌属、链霉菌属和疣孢菌属。基于98- 100%的16 S rRNA基因序列同一性，将这些菌株分为26-56个操作分类单位（OTU）。在98%的序列同一性下，三个OTU似乎代表新的分类群，而九个（35%）仅报告来自海洋环境。其中63株需要海水生长。这些在98%的同一性水平上落入两个OTU中，并且包括一个不能产生气生菌丝并且仅是远亲的OTU（≤ 95.5%16S同一性）与任何先前培养的链霉菌属。与聚酮合酶基因相关的酮合酶结构域的系统发育分析显示序列范围为55至99%，核苷酸的身份，以实验表征的生物合成途径，表明一些可能与新的次级代谢产物的生产。这些结果表明，海洋沉积物从加州海湾港口不同放线菌类群的潜力，以产生新的次生代谢产物。
The Gulf of California is a coastal marine ecosystem characterized as having abundant biological resources and a high level of endemism. In this work we report the isolation and characterization of Actinobacteria from different sites in the western Gulf of California. We collected 126 sediment samples and isolated on average 3.1–38.3 Actinobacterial strains from each sample. Phylogenetic analysis of 136 strains identified them as members of the genera Actinomadura, Micromonospora, Nocardiopsis, Nonomuraea, Saccharomonospora, Salinispora, Streptomyces and Verrucosispora. These strains were grouped into 26–56 operational taxonomic units (OTUs) based on 16S rRNA gene sequence identities of 98–100 %. At 98 % sequence identity, three OTUs appear to represent new taxa while nine (35 %) have only been reported from marine environments. Sixty-three strains required seawater for growth. These fell into two OTUs at the 98 % identity level and include one that failed to produce aerial hyphae and was only distantly related (≤95.5 % 16S identity) to any previously cultured Streptomyces sp. Phylogenetic analyses of ketosynthase domains associated with polyketide synthase genes revealed sequences that ranged from 55 to 99 % nucleotide identity to experimentally characterized biosynthetic pathways suggesting that some may be associated with the production of new secondary metabolites. These results indicate that marine sediments from the Gulf of California harbor diverse Actinobacterial taxa with the potential to produce new secondary metabolites.