Cryo-EM in the study of membrane transport proteins.
Cryo-EM in the study of membrane transport proteins.
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DOI：
10.1002/cphy.c110028
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发表时间：
2012
影响因子：
5.8
通讯作者：
L. Y. Kim;M. Johnson;I. Schmidt-Krey
中科院分区：
文献类型：
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作者：
L. Y. Kim;M. Johnson;I. Schmidt-Krey
低温电子显微镜（cryo-EM）已经发展成为一种广泛使用的方法来了解一系列结构-功能问题，特别是膜蛋白。电子晶体学和单粒子分析的研究提供了丰富的信息，膜转运蛋白。冷冻-EM方法，强调电子晶体学，它产生了最膜转运蛋白的结构信息的任何冷冻-EM技术，在这里进行了描述。二维结晶的方法进行了概述，以及在低温EM标本制备，数据收集和图像处理的进展。所描述的膜转运蛋白结构的例子用于说明在结构理解和方法方面的一些进展。进一步的例子概述了通过电子晶体学和X射线晶体学以及其他补充方法的组合获得的令人印象深刻的结果。
Electron cryomicroscopy (cryo-EM) has evolved as a widely used approach to understand a range of structure-function questions, particularly of membrane proteins. Studies by both electron crystallography and single particle analysis have provided a wealth of information on membrane transport proteins. Cryo-EM methods with an emphasis on electron crystallography, which has yielded the most membrane transport protein structural information of any of the cryo-EM techniques, are described here. Two-dimensional crystallization approaches are outlined, as well as advances in cryo-EM specimen preparation, data collection, and image processing. Examples of membrane transport protein structure described serve to illustrate some of the advances in both structural understanding and methods. Further examples outline impressive results that were obtained by a combination of electron crystallography and X-ray crystallography as well as additional complementary methods.