Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of Tomato spotted wilt virus
Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of Tomato spotted wilt virus
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DOI：
10.1099/vir.0.026708-0
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发表时间：
2011-01-01
影响因子：
3.8
通讯作者：
Rubio, Luis
中科院分区：
文献类型：
--
作者：
Lopez, Carmelo;Aramburu, Jose;Rubio, Luis
番茄斑萎病毒（Tomato spotted wilt virus，TSWV）在世界范围内给许多农作物造成严重的经济损失，并且常常克服用于病害控制的抗性品种。核苷酸和氨基酸序列的比较表明，番茄抗性基因Sw-5赋予可以克服的氨基酸取代C到Y在位置118（C118 Y）或T120 N的TSWV运动蛋白，NSm。系统发育分析表明，C118 Y置换在所研究的菌株中通过趋同进化独立地发生了三次，而T120 N置换是一个独特的事件。对单个密码子的非同义和同义变化率的分析表明，取代C118 Y被正向选择。
Tomato spotted wilt virus (TSWV) causes severe economic losses in many crops worldwide and often overcomes resistant cultivars used for disease control. Comparison of nucleotide and amino acid sequences suggested that tomato resistance conferred by the gene Sw-5 can be overcome by the amino acid substitution C to Y at position 118 (C118Y) or T120N in the TSWV movement protein, NSm. Phylogenetic analysis revealed that substitution C118Y has occurred independently three times in the studied isolates by convergent evolution, whereas the substitution T120N was a unique event. Analysis of rates of non-synonymous and synonymous changes at individual codons showed that substitution C118Y was positively selected.